Effect of integrin ±v²3 blocking on interaction of exosomes with osteoblasts extracellular matrix

• Grant number: 16/22741-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2017
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Heloisa Sobreiro Selistre de Araújo
• Grantee: Anelyse Abreu Cortez
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 13/00798-2 - The extracellular matrix in aging, exercise and in the tumor microenvironment, AP.TEM

Abstract

Metastasis remains as the principal cause of deaths in cancer patients. It's a process involving several inte-related steps, since tumor cell detaching from primary tumor, interaction with extracellular matrix, until it transport, adhesion and establishment of a secondary tumor in organism. Recent researches have gained attention to the importance of cell communication though vesicles during metastasis development, mainly regarding the formation of a metastatic niche. Exosomes are vesicles of 40-100 nm, secreted by organism cells that are involved on the transport of information. It’s believed that during metastasis, exosome secretion by tumor cells has adhesion functions in tumor microenvironment, as well as in distant regions from tumors, preparing in this way the pre-metastatic niche. In this way, the objective of this project é to study the interaction of isolated exosomes from cell culture medium with bone extracellular matrix produced in vitro, and if disintegrin DisBa-01, described in literature as integrin ±v²3 inhibitor is capable to interfere in adhesion of isolated vesicles. (AU)