Enterococcus faecalis: Development of a final irrigant to destroy it, survival analysis of it after endodontic treatment and its ability to stimulate inflammatory cytokines after stress.

• Grant number: 16/25133-1
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): April 01, 2017
• Effective date (End): October 14, 2020
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal researcher: Marco Antonio Hungaro Duarte
• Grantee: Rafaela Fernandes Zancan
• Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated scholarship(s): 18/03554-0 - Influence of different endodontic protocols on the dentin structure and in the adhesive dentin-sealer interface, BE.EP.DR

Abstract

This study consist of three phases. In first step the objective is to develop a new chelating agent, with higher antiseptic capacity. In second step, the persistent bacteria that resist the endodontic treatment employing the better EDTA chelating agent of the first step will be measured by ATP-assay technique. In third step, will be analyzed the effects of stressed Enterococcus faecalis, in planktonic and biofilm form on cytokines production from macrophages. Methods: Step 1: 5 chelating agents will be tested. (N=10) Exp. 1:The smear layer production will be induced on dentin blocks and it reduction analyzed by MEV. Exp. 2 -The antimicrobial action of chelating agents will be tested for 5 minutes against Ent. Faecalis biofilm induced on dentin blocks and measured by confocal and bioimage programs. Exp. 3 - Dentin blocks will be treated with the chelating agents, after, the Ent. Faecalis adhesion to dentin was measured. Exp. 4 - Surface wettability was assessed using the sessile drop method. Exp. 5 - Bovine teeth filled with AH plus will have their roots sectioned, then the push-out test will be performed on the MTS 810 universal testing machine. Exp. 6 - Cytotoxicity will be evaluated by XTT reduction, neutral red and violet crystal evaluation. Step 2: Exp. 1 and 2 - Root canal and intratubular contamination will be make with Ent. Faecalis (N=10) . and treated with 5 protocols. After 3 months the evaluation of persistent micro-organisms will occur by ATP assay method. Step 3: Macrophages production of inflammatory cytokines stimulated by Ent. faecalis stressed on biofilm form will be measured by Elisa test.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAFAELA FERNANDES ZANCAN; JOSÉ BURGOS PONCE; THIAGO JOSÉ DIONISIO; RODRIGO CARDOSO DE OLIVEIRA; RAFAELA ALVES DA SILVA; JUSSARO ALVES DUQUE; MARCO ANTONIO HUNGARO DUARTE. Profile of host cell responses to exposure to stressed bacteria in planktonic; dislodged, and intact biofilm mode. Brazilian Dental Journal, v. 32, n. 3, p. 10-20, Jun. 2021.

ZANCAN, RAFAELA FERNANDES; SOUZA CALEFI, PEDRO HENRIQUE; BATISTA BORGES, MARIANA MACIEL; MILANDA LOPES, MARCELO RIBEIRO; DE ANDRADE, FLAVIANA BOMBARDA; VIVAN, RODRIGO RICCI; HUNGARO DUARTE, MARCO ANTONIO. Antimicrobial activity of intracanal medications against both Enterococcus faecalis and Candida albicans biofilm. MICROSCOPY RESEARCH AND TECHNIQUE, v. 82, n. 5, p. 494-500, MAY 2019. Web of Science Citations: 0.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ZANCAN, Rafaela Fernandes. Enterococcus faecalis: development of a final irrigant to destroy it, analysis of its survival after endodontic treatment and its ability to stimulate pro-inflammatory cytokines after stressed. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.