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Evaluation of intestinal dysbiosis in patients with rheumatoid arthritis and correlation with Th1 and Th17 cytokines

Grant number: 17/03463-2
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2017
Effective date (End): March 31, 2018
Field of knowledge:Biological Sciences - Immunology
Principal Investigator:Gislane Lelis Vilela de Oliveira
Grantee:Guilherme Siqueira Pardo Rodrigues
Host Institution: Faculdade de Ciências da Saúde de Barretos Dr Paulo Prata (FACISB). Barretos , SP, Brazil


In humans, over one hundred trillion microorganisms, mainly bacteria, colonize the oral-gastrointestinal tract, and the vast majority of these reside in the distal gut and was termed microbiota. The most important microbiota's contributions for the host include carbohydrates digestion and fermentation, vitamin production, mucosal-associated lymphoid tissues development, immune responses polarization and prevent pathogens colonization. However, when this mutualistic relationship between host and microbiota is interrupted, the intestinal microbiota may cause or contribute to the development of infectious diseases, inflammatory and autoimmune diseases. Therefore, the aim of this study is to evaluate intestinal dysbiosis in patients with rheumatoid arthritis (RA) and to correlate with plasma concentrations of Th1 and Th17 cytokines. Patients and control subjects will be included in the health center from Health Department from Barretos. The study was approved by the Ethics Committee (1269/2016), and patients and controls will sign the informed consent and answer a socio-epidemiological questionnaire. Fecal samples are collected by own individuals or families in universal collectors. Clinical data such as deformities due to tendon rupture and joint erosions, involvement of the cervical spine, extra-articular manifestations, neurological, rheumatoid factor, anti-CCP, erythrocyte sedimentation rate, C-reactive protein, composite indexes of disease activity (ICAD), measures of functional capacity (HAQ) and drug treatment will be recorded. Bacterial DNA is extracted by using commercial kit and the microbiota characterization will be performed by real-time PCR. The quantification of Th1 and Th17 cytokines will be done by ELISA assays. The results of the intestinal microbiota of patients and controls will be analyzed by using Mann-Whitney test and correlations of microbiota results with clinical and cytokines data will be assessed by Spearmans' test. We expect to find differences in the composition of the intestinal microbiota of patients with RA compared with healthy subjects and possible correlations with clinical data and Th1 and Th17 cytokines. Additional studies are necessary, and possibly in the future, immunomodulatory probiotics may aid in the treatment of RA. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, GUILHERME S. P.; CAYRES, LEONARDO C. F.; GONCALVES, FERNANDA P.; TAKAOKA, NAUYTA N. C.; LENGERT, ANDRE H.; TANSINI, ALINE; BRISOTTI, JOAO L.; SASDELLI, CAROLINA B. G.; DE OLIVEIRA, V, GISLANE L.. Detection of Increased Relative Expression Units of Bacteroides and Prevotella, and Decreased Clostridium leptum in Stool Samples from Brazilian Rheumatoid Arthritis Patients: A Pilot Study. MICROORGANISMS, v. 7, n. 10, . (17/03463-2)

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