Interaction between phosphate fertilizer and soil microbial diversity in the plant colonization by arbuscular mycorrhizal fungi

• Grant number: 16/21596-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2017
• End date: February 21, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Fernando Dini Andreote
• Grantee: Bruna Arruda
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 18/14373-7 - Microbial community interaction and its effect on the phosphorus cycling in native soils from Brazil and United Kingdom, BE.EP.DR

Abstract

In highly weathered tropical soils, phosphorus (P) is not widely available, becoming the symbiotic associations with mycorrhizal fungi (AMF) as a beneficial nutrient acquisition strategy. In general, microbial community interacts strongly with FMA, affecting mycorrhizal colonization in host plants. However, there is a lack of information about the interaction of microbial community with AMF under different phosphorus availabilities. The objective of this project is to understand the dynamics of different soil microbial communities and the FMA under phosphate application. First, there will be an experiment to select two host plants starting from six plant species, inoculated with three AMF species: Rhizophagus clarus (= Glomus clarum); Dentiscutata heterogama (=Scutellospora heterogama) and Acalouspora scrobiculata, evaluating mycorrhizal colonization by qPCR technique (quantitative PCR). Second, the two selected plants will be inoculated with three AMF species in systems containing different levels of microbial community diversity, induced by: i) dilution (10-1 10-3 or 10-6); and ii) by heat treatment (50, 80 or 100 degree Celsius for one hour), where the mycorrhizal colonization rate will be evaluated. Finally, six experiments (2x3) will be conducted involving: the two selected plants and the three AMF species, under five dosages of P, via triple superphosphate. The variables to analyze are: mycorrhizal colonization rate by plant species, structure of bacterial and fungal communities, P content in plant tissue and soil, in order to evaluate if P fertilization can minimize the impact of mycorrhizal colonization by the reduction of microbial diversity, and if there is any plant assimilation of P added.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARRUDA, BRUNA; GEORGE, PAUL B. L.; ROBIN, AGNES; DE L. C. MESCOLOTTI, DENISE; HERRERA, WILFRAND F. B.; JONES, DAVEY L.; ANDREOTE, FERNANDO D.. Manipulation of the soil microbiome regulates the colonization of plants by arbuscular mycorrhizal fungi. MYCORRHIZA, v. 31, n. 5, AUG 2021. (16/21596-7)

ARRUDA, BRUNA; RODRIGUES, YASMIN FLORENTINO; HERRERA, WILFRAND FERNEY BEJARANO; ROBIN, AGNES; COTTA, SIMONE RAPOSO; ANDREOTE, FERNANDO DINI. Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root. Journal of Microbiological Methods, v. 192, p. 4-pg., 2022-01-01. (16/21596-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ARRUDA, Bruna. Soil microbiome manipulation and its effect on the soil-plant interface. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.