THE ROLE OF SOCS AND STATS PROTEINS ON THE DIFFERENTIATION OF TCD4+ NAIVE CELLS IN THE CHRONIC OBSTRUCTIVE PULMONARY DISEASE (COPD) DEVELOPMENT: COMPARATIVE ANLYSIS BETWEEN LOCAL AND SYSTEMIC IMMUNOLOGICAL RESPONSE

• Grant number: 16/21408-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2017
• End date: September 30, 2020
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Fernanda Degobbi Tenorio Quirino dos Santos Lopes
• Grantee: Juliana Dias Lourenço
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Smoking is considered the main risk factor for development of Chronic Obstructive Pulmonary Disease (COPD), however other intrinsic factors to the patient as well as the role of innate and adaptative immunity, are also involved in this process. In a previous study of our group, we demonstrated that the decrease of T regulatory (Treg) density cells, associated to lower density of IL-10 and TGF-² cells in obstructive smokers compared to non obstructive smokers, could be one of the mechanisms involved at the COPD progression. Aims: To evaluate the mechanisms involved at the TCD4+ naive cells differentiation to its different subtypes, especially the Th17 and Treg cells, considering the role of SOCS and STATS in this events, in obstructive (COPD) and non obstructive smokers, comparing the local and systemic immunological response. Methods: Patients submitted to pulmonary resection of primary metastatic tumor will be study and divided into two groups: COPD Group and Non Obstructive Smokers Group. It will be evaluated the interleukins -6, -10, -17 and TGF-² in the lungs and blood plasma by ELISA, to verify the local and systemic response. Also in the lungs, it will be evaluated the positive cells for IL-17 (Th17 profile), Foxp3 (Treg) and STATS and SOCS (total and phosphorylated STAT 3 and 5, SOCS 1 and 3) by double staining. We will perform quantitative analysis of total and phosphorylated STAT 3 and 5, SOCS 1 and 3 by immunoblotting, and we will evaluate the gene expression of STAT 3 and 5, SOCS 1and 3 by real time PCR in patients lung and blood plasma.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOURENCO, JULIANA D.; TEODORO, WALCY R.; BARBEIRO, DENISE F.; VELOSA, ANA PAULA P.; SILVA, LARISSA E. F.; KOHLER, JULIA B.; MOREIRA, ALYNE R.; AUN, V, MARCELO; DA SILVA, ISADORA C.; FERNANDES, FREDERICO L. A.; et al. Th17/Treg-Related Intracellular Signaling in Patients with Chronic Obstructive Pulmonary Disease: Comparison between Local and Systemic Responses. CELLS, v. 10, n. 7, JUL 2021. (16/17817-8, 16/21408-6)

LOURENCO, JULIANA DIAS; DE FRANCA SILVA, LARISSA EMIDIO; DE GENARO, ISABELLA SANTOS; ITO, JULIANA TIYAKI; PIETROBON, ANNA JULIA; SATO, MARIA NOTOMI; GROSS, JEFFERSON LUIZ; NEGRI, ELNARA MARCIA; BARBEIRO, DENISE FREDIANI; ROSOLIA TEODORO, WALCY PAGANELLI; et al. Intracellular mechanisms of Th17/Treg differentiation in mild and moderate COPD patients. European Respiratory Journal, v. 54, p. 2-pg., 2019-09-28. (16/17817-8, 16/21408-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LOURENÇO, Juliana Dias. Study of STAT and SOCS proteins expression in chronic obstructive pulmonary disease development: comparison between the local and systemic immune responses. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.