Characterization of microvesicles and mesangial cells derived-exosomes stimulated by glucose and evaluation of their possible role in cell communication

• Grant number: 17/00250-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2017
• End date: June 30, 2020
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Mirian Aparecida Boim
• Grantee: Antônio da Silva Novaes
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 15/23345-9 - MicroRNAs, extracellular vesicles and stem cells: physiology, pathophysiological role and therapeutic potential in renal diseases, AP.TEM

Abstract

Exosomes are extracellular vesicles involved in intercellular communication under physiological and pathophysiological conditions, altering the function of target cells. Substances carried by these microstructures, whose content is determined by the secreting cell, confer this capacity. Stimuli and/or insults to the cells producing exosomes are capable to modify the amount and the cargo of secreted macrovesicles that in turn can alter the function of target cells. The macrovesicles and exosomes can constitute a pathway to disseminate factors with pathophysiological potential. Mesangial Cells (MC) have a relevant physiological role in the regulation of glomerular filtration, and their dysfunction may contribute to functional changes in the glomerulus contributing to typical glomerular sclerosis of Diabetic Nephropathy (DN). Thus, the objectives of this project include an in vitro analysis of the ability of MC in culture to secrete exosomes. Also to evaluate their content under standard physiological conditions and after stimulation with high glucose concentration concerning the presence of inflammatory and profibrotic molecules as well as the components of the renin angiotensin system, classically involved in the pathophysiology of DN. We also intend to assess whether high glucose stimulated MC derived-exosomes would be able to alter the function of normal target MC cells, and of distinct cell lineages such as podocytes, endothelial cells and tubular cells (in vitro) or alter renal function (in vivo) by injection (subcapsular) of these vesicles in the kidneys of healthy animals. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

NOVAES, ANTONIO DA SILVA; BORGES, FERNANDA TEIXEIRA; MAQUIGUSSA, EDGAR; VARELA, VANESSA ARAUJO; SALLES DIAS, MARCOS VINICIOS; BOIM, MIRIAN APARECIDA. Influence of high glucose on mesangial cell-derived exosome composition, secretion and cell communication. SCIENTIFIC REPORTS, v. 9, APR 18 2019. (15/23345-9, 17/00250-8)