Co-inoculation of Rhizobium and Azospirillum in common bean crop

• Grant number: 16/07591-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2017
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Adalton Mazetti Fernandes
• Grantee: Amanda Prado Gilabel
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Among the nutrients required by the common bean (Phaseolus vulgaris L.), nitrogen (N) is the most taken up. Nitrogen fertilizers, a major source of N for crop, have high economic and energetic costs, low efficiency of use by plants, in addition to the environmental risk. Thus, there is great interest in strategies aimed at reducing the use of fertilizers and consequently improvements in sustainability in agricultural areas. In that sense, a technique that is beginning to be explored in the bean crop is co-inoculation, which is the association of Azospirillum with the Rhizobium. It is possible that some bacteria, among which those of the genus Azospirillum brasilense, benefit plant growth by a combination of various mechanisms. The aim of this study is to evaluate the effect of co-inoculation on root growth, nodulation, N accumulation and grain yield of common bean, and understand how technology can benefit the crop performance. The research will consist of two experiments in field conditions, during the two growing season ("spring-summer" and "summer-autumn"), in Experimental Farm Lageado, in College of Agricultural Sciences - UNESP, in Botucatu - SP. The experiments will be conducted in the design of randomized blocks with four replications. The treatments are: T0: Control absolute (without inoculation and without N fertilization); T1: Control with N (without inoculation and 80 kg ha-1 N); T2: Rhizobium tropici inoculation 2.5 x 106 cells seed-1; T3: Azospirillum brasilense inoculation 2.5 x 105 cells seed-1; T4: A. brasilense inoculation 5.0 x 105 cells seed-1; T5: co-inoculation R. tropici 2.5 x 106 cells seed-1 + A. brasilense 2.5 x 105 cells seed-1; T6: co-inoculation R. tropici 2.5 x 106 cells seed-1 + A. brasilense 5.0 x 105 cells seed-1. In R6 stage common bean plants will be assessed for relative chlorophyll index, leaf diagnoses, number and dry weight of nodules, N-ureido content, root growth, leaf area, dry matter of root and shoot, concentration and accumulation of nutrients in the shoot. On the occasion of the plant cycle closure will be evaluated yield components, grain yield and protein concentration in grains. The data will be submitted to analysis of variance. The treatment means are compared by Tukey's test at 5% probability. In this study we know that co-inoculation stimulates the growth of the root surface and thereby increases the volume of soil explored by them, and also this technique enhances the biological N2-fixation by Rhizobium, through increased nodulation and / or the activity nodule. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GILABEL, Amanda Prado. Co-inoculação de rhizobium e azospirillum e adubação nitrogenada na cultura do feijão comum. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agronômicas. Botucatu Botucatu.