Molecular characterization, sensitivity profile and biofilm production from Escherichia coli isolated from bulk tanks

• Grant number: 17/07246-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: May 01, 2017
• End date: February 29, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Helio Langoni
• Grantee: Samea Fernandes Joaquim
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 15/19688-8 - E. coli, Klebsiella pnemoniae and Enterococcus spp: impact of virulence factors in bovine mastitis and public health concern, AP.TEM

Abstract

There is a worldwide concern about the quality of milk offered for consumption. The presence of coliforms in the milk within the established limit, guarantees the effectiveness of the inactivation of the microorganisms during pasteurization. However, when there are failures in the pasteurization of milk, or even when there is consumption of the raw product, cases of diseases in humans may occur. Molecular studies to identify strains of Escherichia coli in expansion tanks are practically incipient in Brazil, as well as the clonal profile of the isolates. The increase of resistance of the pathogens to the antimicrobials points to the need to investigate the sensitivity profile of bacteria isolated from milk from expansion tanks. Thus, the present study intends to identify the E. coli profile presents in milk expansion tanks as well as genes associated with virulence factors, genes involved in the formation of biofilms and the search of genes of isolates producing ²-lactamase enzyme (ESBL), as well as phenotypic studies of antimicrobial multiresistance and formation of biofilms in different materials. Thus, it is desired to deepen the knowledge of this pathogen, in order to enable control strategies to improve the quality of milk from expansion tanks, aiming at public health. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

JOAQUIM, Samea Fernandes. Molecular characterization, sensitivity profile and biofilm production from bulk tank milk Escherichia coli.. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.