Impact of the herbivorous insect attack in the exudates and microorganism community of the Arabidopsis thaliana rhiszosphere

• Grant number: 17/05465-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 01, 2017
• End date: August 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Márcio de Castro Silva Filho
• Grantee: Márcia Leite Mondin
• Supervisor: Jorge Manuel Vivanco
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Institution abroad: Colorado State University, United States
• Associated to the scholarship: 16/18001-1 - Possible effect of insect herbivores in changing the rhizosphere microorganisms composition from the protein differential secretion and phytochemicals in Arabidopsis thaliana, BP.DR

Abstract

The plant root systems role in the physiological mechanisms that ensure soil nutrient uptake and retention as functional organic reserve in some plant families is well established. However, we seek knowledge of the secondary metabolites and small molecules found in plant exudates that play an important role in the interaction with soil microorganisms and may induce resistance throughout the plant. Understand as the exudate components act allowing a selection of beneficial microorganisms in contrast of to herbivore attack is key in the establishment of a relationship with plant resistance; and it is the main aim of this project. The study will be performed at Colorado State University, for characterize the secondary metabolites and small molecules that are exuded by A. thaliana root system in a well-established experimental system that will allow know if there is a selection of microorganism after the insect attack of the genus Trichoplusia. The results will be used as a reference to compare with the experiments carried out in Brazil using A. thaliana grown in a comercial substrate for gardening and submitted to herbivore attack of the insect Spodoptera frungiperda. The well-established experimental system study will be conducted with soil collected at Michigan Extension Station, Port of Benton, MI / USA, where Arabidopsis thaliana grows naturally. Despite the fact of the soil from Michigan is well characterized to its microorganism community, all the soil used to the experiments will be analysed before sowing to establish e initial microorganism community structure that will be used to be compared with those found after insect attack. Rhizosphere microorganism community will be quantitatively characterized by TRFLP and qPCR. The elucidation of the microorganism-exudates-plant interaction mechanism may reveal new future strategies aiming to increase the resistance of the crops and, consequently, to increase a food productivity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEITE-MONDIN, M.; DILEGGE, M. J.; MANTER, D. K.; WEIR, T. L.; SILVA-FILHO, M. C.; VIVANCO, J. M.. The gut microbiota composition of Trichoplusia ni is altered by diet and may influence its polyphagous behavior. SCIENTIFIC REPORTS, v. 11, n. 1, MAR 11 2021. (16/18001-1, 14/50275-9, 17/05465-2)