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Gene expression of immunity markers in keloid

Grant number: 16/20215-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2017
Effective date (End): May 31, 2018
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Alfredo Gragnani Filho
Grantee:Tatiana Figueiredo Veras
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Keloid is a dermal injury that surpasses the margins of the original injury, it continues to grow over time and doesn't regress spontaneously. It appears more frequently in certain anatomic regions that were submitted to skin trauma. After injury, the body initiates an immediate and consistent damage repair process that leads to the formation of a scar. When the repair process is unbalanced, the scar may be affected, resulting in keloid formation. This process is associated to several immunity factors such as cytokines, growth factors, macrophages, neutrophils and lymphocytes. This way, a new perspective into the keloid pathogenesis is introduced, a perspective that involves an abnormal immune response to dermal injury. Given the correlations between the immune process and the formation of keloid, it is important to study the gene profile related to the immediate and adaptive immune response of patients with keloid in specific areas, such as the ear lobe. Objective: to evaluate the gene expression of immunity markers in keloid. Method: the study will include twelve (12) patients with keloid and twelve (12) in control group. The keloid samples will be obtained by excision at the subcutaneous tissue level. The RNA will be extracted from skin samples following PCR array. For the study, 84 genes will be analyzed for human genes relevant to the human immediate and adaptive immune response - Human Innate & Adaptive Immune Responses (PAHS-052ZC). (AU)