Evaluation of angiogenesis in xenografts collected from bovine bone implanted at intracorporeal sites of rabbits (Oryctolagus cuniculus) as an in vivo model of bioreactor

• Grant number: 17/04142-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2017
• End date: February 28, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Paola Castro Moraes
• Grantee: Gabriel Luiz Montanhim
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

There is an increasing demand for natural or synthetic materials that help in tissue recovery, including those in consequence of high energy trauma, postoperative complications or bone neoplasias. As analternative to correct these failures, graft sandbones are used, in which autologous grafts present the best tissue responses in terms of biocompatibility. Due to the low amount of autologous grafts as well as surgical morbidity on the site of collection, there is a growth in the number of researches on the use of xenologous grafts, but usually of bovine origin. However, to potentiate the production and quantity of neoformed cells from the graft, the use of bioreactors be comes essential. In this way, xenologous grafts are implanted in bioreactor animals which, depending on the site of implantation and the inflammatory response caused, are stimulated to produce new cells. Thus, a quantity of new tissue, now with the desired characteristics, is produced, in order to be implanted at the site of bone failure, providing increased tissue repair.The omentum, for example, is an organ that due to its neoangiogenic capacity, can help in the cellular enrichment in the new lyimplanted tissue. The objective of this study was to evaluate the angiogenic and osteoinductive capacity of xenogenenous grafts implanted in different intracorporeal sites of rabbits, considered as bioreactors. For this, 30 rabbits are used and distributed in six groups, that receives xenogenous implants at three different intracorporeal sites. Macroscopic and histopathological analyzes are made of each sample collected. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

G.L. MONTANHIM; M.T.S. SILVA; M.E.B.A.M. CONCEIÇÃO; T.S. ROCHA; L.M. YAMASHIRO; J.O. RIBEIRO; A. HATAKA; P.R.R. MOREIRA; B.W. MINTO; L.G.G.G. DIAS; et al. [Avaliação da angiogênese em xenoenxertos bovinos implantados em sítios intracorpóreos de coelhos como modelos de biorreatores in vivo]. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 73, n. 5, p. 1067-1075, 2021-11-05. (17/04142-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MONTANHIM, Gabriel Luiz. Evaluation of angiogenesis in xenografts collected from bovine bones implanted at intracorporeal sites of rabbits (Oryctolagus cuniculus) as an in-vivo bioreactor model. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.