Proteomic and post-translation modification characterization of Leishmanias L. (V.) braziliensis, L. (L.) amazonensis and L. (L.) chagasi using a shotgun protemics approaches.

• Grant number: 16/23689-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2017
• End date: September 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Giuseppe Palmisano
• Grantee: Joyce Silva Saad
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications, AP.JP

Abstract

Leishmaniasis is a tropical diseases present in more than 98 countries, being the second most common parasitosis after malaria, which results in 20,000-30,000 deaths annually. It is caused by the flagellate protozoan of the genus Leishmania, which comprises more than 20 species that lead to different manifestations of Leishmaniasis, as visceral leishmaniasis, cutaneous, mucocutaneous and cutaneous-diffuse.Often asymptomatic, the Leishmaniasis are difficult to diagnose, being the immunological and molecular methods the most used. Proteomics, which uses mass spectrometry as main methodology, has aided the immunological and molecular methods to better understand the biological role during the illness and the virulence-pathogenicity and pathogen-host interactions.This project aims to compare the proteome and their post-translational modifications (phosphorylation and glycosylation) of three species of Leishmania: L. (V.) braziliensis, L. (L.) amazonensis and L. (L.) chagasi. This analysis will be essential to generate a proteomic map that will provide: 1) database for the scientific community, 2) the re-annotation of genome of these species with proteogenomic metholodogy and 3) the possibility of better comprehend the intracellular signaling and its relationship with the form and manifestations of the disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MULE, SIMON NGAO; SAAD, JOYCE SILVA; FERNANDES, LIVIA ROSA; STOLF, BEATRIZ S.; CORTEZ, MAURO; PALMISANO, GIUSEPPE. Protein glycosylation inLeishmaniaspp.. MOLECULAR OMICS, v. 16, n. 5, p. 407-424, OCT 1 2020. (20/04923-0, 18/15549-1, 16/23689-2, 17/04032-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SAAD, Joyce Silva. Identification of the proteomic variability of the promastigote form of the species Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis, and Leishmania (Leishmania) chagasi, using shotgun proteomics approaches.. 2019. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.