Comparative study of the use of reactor, ultrasound and maceration in the preparation of standardized extracts of Alternanthera paronychioides and evaluation of antioxidant activity

• Grant number: 17/09781-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2017
• End date: June 30, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Marcos José Salvador
• Grantee: Fernando Henrique Bosso
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The objective of this project is to perform the comparative study of the use of reactor, ultrasound and maceration as extraction methods for the preparation of standardized extracts in total flavonoids of Alternanthera paronychioides (Amaranthaceae), a Brazilian plant with potential antioxidant activity and antidiabetics. Therefore, it will be used as extractive liquids, distilled water and ethanol, and different extraction times for the same amount of vegetable powder and extractive liquid. The antioxidant activity in vitro (B-carotene assays, DPPH radical reduction and ORAC-FL) of the extracts will be evaluated and the active extracts will be quantified with total soluble phenolics using the Folin- Ciocalteu. Thus, this study may allow the development of procedures to obtain standardized extracts of A. paronychioides, in addition to evaluating whether the use of jacketed reactor and ultrasound improves the process of preparation of bioactive extracts, with maintenance of mass weight of the preparations obtained and reducing the time of extraction when compared to the classic method of maceration. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KASSUYA, ROBERTO MIKIO; DOS SANTOS, ELISANGELA; BOSSO, FERNANDO HENRIQUE; PEDROSO, TAISE FONSECA; MARINHO, JANE VASCONCELOS NEVES; SALVADOR, MARCOS JOSE; KASSUYA, CANDIDA APARECIDA LEITE; GASPAROTTO JUNIOR, ARQUIMEDES. Anti-inflammatory Properties of Ethanolic Extract and 2 `'-O-beta-d-Glucopyranosyl-vitexin Obtained from Alternanthera tenella Colla Whole Plant. Inflammation, v. 44, n. 4, p. 1540-1552, AUG 2021. (17/09781-6, 15/03726-8, 06/06079-4)