Proteomics: a tool to investigate the composition and function of HDL in hyperlipidemia

• Grant number: 17/07725-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2017
• End date: December 31, 2021
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Graziella Eliza Ronsein
• Grantee: Amanda Ribeiro Martins da Silva
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The excess of lipids in the diet and its metabolic consequences are a worldwide epidemic. Only in Brazil, more than half the population is overweight or obese. Obesity, metabolic syndromes and dyslipidemias are strongly associated with the development of cardiovascular diseases, one of the main causes of overall mortality. Hyperlipidemic individuals present a variety of structural and functional alterations in plasma lipoproteins that contribute to arterial damage. Treatments to lower low-density lipoprotein (LDL) cholesterol save thousands of lives each year, but the residual risk remains high. On the other hand, high-density lipoprotein cholesterol (HDL) exhibits cardioprotective effects, which seem to be impaired when it becomes dysfunctional. The protective effects of HDL are due to the mobilization of excess cholesterol from arterial walls macrophages. HDL is a heterogeneous collection of particles and the molecules that determine its functionality are largely unknown. In this sense, the present project aims to understand the mechanisms that generate dysfunctional HDL in an animal model of hyperlipidemia. To this end, the multifactorial approach of proteomics will be employed to investigate the complex metabolism of HDL. Altered lipoprotein proteomics of mice submitted to a hyperlipidic diet will be correlated with functional assays. This work may contribute significantly to the understanding of pathways that generate dysfunctional lipoproteins under conditions of hyperlipidemia. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA JUNIOR, DOUGLAS RICARDO; MARTINS SILVA, AMANDA RIBEIRO; ROSA-FERNANDES, LIVIA; REIS, LORENNA ROCHA; ALEXANDRIA, GABRIELLY; BHOSALE, SANTOSH D.; GHILARDI, FABIO DE ROSE; DALCOQUIO, TALIA FALCAO; BERTOLIN, ADRIADNE JUSTI; NICOLAU, JOSE CARLOS; et al. HDL proteome remodeling associates with COVID-19 severity. JOURNAL OF CLINICAL LIPIDOLOGY, v. 15, n. 6, p. 796-804, NOV-DEC 2021. (15/26722-8, 16/00696-3, 20/04923-0, 19/25702-4, 17/07725-1, 18/15549-1, 18/18257-1, 13/07937-8)

SILVA, AMANDA R. M.; TOYOSHIMA, MARCOS T. K.; PASSARELLI, MARISA; DI MASCIO, PAOLO; RONSEIN, GRAZIELLA E.. Comparing Data-Independent Acquisition and Parallel Reaction Monitoring in Their Abilities To Differentiate High-Density Lipoprotein Subclasses. JOURNAL OF PROTEOME RESEARCH, v. 19, n. 1, p. 248-259, JAN 2020. (17/07725-1, 16/00696-3, 13/07937-8)

SOUZA JUNIOR, DOUGLAS RICARDO; SILVA, AMANDA RIBEIRO MARTINS; RONSEIN, GRAZIELLA ELIZA. Strategies for consistent and automated quantification of HDL proteome using data-independent acquisition. Journal of Lipid Research, v. 64, n. 7, p. 14-pg., 2023-07-03. (17/07725-1, 16/00696-3, 13/07937-8, 19/25702-4)

SANTANA, MONIQUE F. M.; LIRA, AECIO L. A.; PINTO, RAPHAEL S.; MINANNI, CARLOS A.; SILVA, AMANDA R. M.; SAWADA, MARIA I. B. A. C.; NAKANDAKARE, EDNA R.; CORREA-GIANNELLA, MARIA L. C.; QUEIROZ, MARCIA S.; RONSEIN, GRAZIELLA E.; et al. Enrichment of apolipoprotein A-IV and apolipoprotein D in the HDL proteome is associated with HDL functions in diabetic kidney disease without dialysis. LIPIDS IN HEALTH AND DISEASE, v. 19, n. 1, p. 14-pg., 2020-09-14. (16/00696-3, 18/00172-0, 17/07725-1, 16/15603-0, 15/21072-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Amanda Ribeiro Martins da. Proteomics: a tool to investigate of composition and function of HDL in hyperlipidemia. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.