Alleviating the impact of water deficit on sugarcane photosynthesis by managing nitrogen source

• Grant number: 17/11279-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2017
• End date: July 31, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Rafael Vasconcelos Ribeiro
• Grantee: Maria Dolores Pissolato
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Water and nitrogen are some of the most limiting factors in world agricultural production, being both factors strongly associated with plant photosynthesis. Water deficit reduces CO2 assimilation as a consequence of decreases in stomatal conductance and metabolic impairment. Changes in nitrogen metabolism under water deficit are poorly understood in C4 plants and any information is still fragmented. It is known that the radical nitric oxide (NO) is an important signaling molecule in several biological systems, protecting cells by reducing free radicals formed under oxidative stress. In such scenario, the influence of nitrogen nutrition on physiological responses of sugarcane to water deficit will be evaluated, considering the photosynthetic activity, antioxidant metabolism and plant growth. The hypothesis to be tested is that plants that receive nitrate as source of nitrogen will have higher activity of nitrate reductase and produce more NO under water deficit, reducing the oxidative damage caused by ROS and favoring the photosynthetic metabolism under low water availability. Sugarcane plants will be supplied with different sources of N (ammonium or nitrate) and subjected to water deficit by adding PEG-8000 to the nutrient solution (osmotic potential of -0.75 MPa). The experimental design will be in randomized complete blocks, with five replicates. Data will be subjected to the analysis of variance and mean values compared by the Tukey test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PISSOLATO, MARIA D.; SILVEIRA, NEIDIQUELE M.; MACHADO, EDUARDO C.; ZAMBROSI, FERNANDO C. B.; SODEK, LADASLAV; RIBEIRO, V, RAFAEL. Photosynthesis and biomass accumulation in young sugarcane plants grown under increasing ammonium supply in nutrient solution. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. 31, n. 3, p. 401-411, SEP 2019. (17/11279-7)

PISSOLATO, MARIA DOLORES; SILVEIRA, NEIDIQUELE MARIA; PRATAVIERA, PAULA JOYCE CARRENHO; MACHADO, EDUARDO CARUSO; SEABRA, AMEDEA BAROZZI; PELEGRINO, MILENA TREVISAN; SODEK, LADASLAV; RIBEIRO, RAFAEL V.. Enhanced Nitric Oxide Synthesis Through Nitrate Supply Improves Drought Tolerance of Sugarcane Plants. FRONTIERS IN PLANT SCIENCE, v. 11, JUN 30 2020. (18/08194-2, 17/11279-7, 17/05029-8)

SEABRA, AMEDEA B.; SILVEIRA, NEIDIQUELE M.; RIBEIRO, RAFAEL, V; PIERETTI, JOANA C.; BARROSO, JUAN B.; CORPAS, FRANCISCO J.; PALMA, JOSE M.; HANCOCK, JOHN T.; PETRIVALSKY, MAREK; GUPTA, KAPUGANTI J.; et al. Nitric oxide-releasing nanomaterials: from basic research to potential biotechnological applications in agriculture. NEW PHYTOLOGIST, v. 234, n. 4, p. 7-pg., 2022-03-29. (20/03646-2, 12/19167-0, 17/11279-7, 18/08194-2, 19/15095-3, 15/21546-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PISSOLATO, Maria Dolores. Amenizando os efeitos do déficit hídrico em cana-de-açúcar com a nutrição nitrogenada. 2019. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.