Evaluation of biological and mechanical properties of a reliner after immersion in different liquid disinfectant soaps

• Grant number: 17/02141-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2017
• End date: July 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Janaina Habib Jorge
• Grantee: Isadora Ferreira Malavolta
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The objective of this study will be to evaluate some biological (ability to reduce the biofilm of Candida albicans and cytotoxicity) and mechanical (roughness and hardness) properties of a reliner after immersion in different liquid disinfectant soaps. Samples of the material (Tokuyama Rebase Fast II) will be made from circular metal device with 14 mm diameter and 1.2 mm thickness. Then, the samples will be divided into groups according to the type of disinfectant solution: AD: the properties of the samples will be evaluated after immersion in distilled water at 37 oC (control group); SD: the properties of the samples will be evaluated after immersion in Dettol liquid soap; SP: the properties of the samples will be evaluated after immersion in Protex liquid soap; SL: the properties of the samples will be evaluated after immersion in Lifebuoy liquid soap. The minimum inhibitory concentrations of disinfectant soaps for Candida albicans (established in previous studies) will be used for immersion of the samples. After immersion in the different groups, the properties of biofilm reduction capacity of Candida albicans, cytotoxicity, hardness and roughness will be evaluated. For biological tests (ability to reduce Candida albicans biofilm and cytotoxicity) the samples will be immersed in the disinfectant solutions for 8 hours. For the mechanical tests (hardness and roughness) the samples will be immersed in the disinfectant solutions for 0, 7, 15 and 30 days, with daily immersion cycles, for 8 hours at room temperature, followed by immersion in distilled water for another 16 hours at 37 oC, simulating the night disinfection of the dentures, the disinfectant solution and the distilled water being changed daily. The results will be submitted to the most appropriate statistical method and the level of significance of 5% will be selected (± = 0.05). In addition, the cytotoxicity test results will be compared to the negative control and solutions in the different experimental groups will be classified according to the cytotoxic effect in scores of 0 to 3.