In vitro analysis of mast cells and renin-angiotensin system role of spontaneously hypertensive animals osteoblasts and osteoclasts

• Grant number: 17/05873-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2017
• End date: January 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Sandra Helena Penha de Oliveira
• Grantee: Sabrina Cruz Tfaile Frasnelli
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated research grant: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach, AP.TEM

Abstract

Periodontal inflammation is associated with many systemic diseases, being called periodontal medicine, among them, cardiovascular diseases, diabetes (DM) and osteoporosis. Periodontitis (PD) is an inflammatory and infectious process of the ligament and alveolar bone that support the teeth where the release of inflammatory mediators occurs. Periodontitis involves local involvement of the renin-angiotensin system (RAS), since angiotensin acts on inflammation. Spontaneously hypertensive rats (SHR) have been used as genetic model of hypertension (DC). These animals are characterized by high blood pressure, significant changes in bone metabolism and a high activation of the sympathetic nervous system. Mast cells (MCs) play a fundamental role since they act in the tissue repair and in the defense of the organism. MCs have been observed in both the normal and inflamed periodontium, thus, it is assumed that MCs may participate in a possible inflammatory response, which may result in destruction and /or tissue and bone protection. Many studies have proposed that specific proteases (tryptase and chymase) and inflammatory mediators released by MCs are involved in the tissue destruction present in PD, but the mechanisms involved in this process are still unknown. MicroRNAs (miRNAs) play an important role in the regulation of bone metabolism and the immune-inflammatory response; however, the role of miRNAs in periodontal inflammation and bone metabolism in hypertensive animals has not yet been fully elucidated. The purpose of the present study is to investigate the role of MCs and RAS on bone metabolism in SHR submitted to experimental periodontitis. The methodology includes evaluating the role of MCs, miRNAs and RAS involved in osteoblastic and osteoclastic activity in cell culture assays. The prediction is to gain new insights into the response of miRNAs and RAS related to systemic and local mechanisms in periodontal disease and hypertension in relation to changes in bone metabolism. These data will contribute to the development of new drug therapy targets for these diseases.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PENHA OLIVEIRA, SANDRA HELENA; BALERA BRITO, VICTOR GUSTAVO; TFAILE FRASNELLI, SABRINA CRUZ; RIBEIRO, BIANCA DA SILVA; FERREIRA, MILENA NUNES; QUEIROZ, DAYANE PRISCILLA; BELTAN, CARLUCI TAIS; LARA, VANESSA SOARES; SANTOS, CARLOS FERREIRA. Aliskiren Attenuates the Inflammatory Response and Wound Healing Process in Diabetic Mice With Periodontal Disease. FRONTIERS IN PHARMACOLOGY, v. 10, JUL 4 2019. (18/04989-0, 17/05873-3, 18/04476-3, 15/03965-2)

BARRETO, AYNA EMANUELLI ALVES; BRITO, VICTOR GUSTAVO BALERA; PATROCINIO, MARIANA SOUSA; BALLASSONI, BEATRIZ BABETO; FRASNELLI, SABRINA CRUZ TFAILE; OLIVEIRA, SANDRA HELENA PENHA. beta 1-adrenergic receptor but not beta 2 mediates osteogenic differentiation of bone marrow mesenchymal stem cells in normotensive and hypertensive rats. European Journal of Pharmacology, v. 911, NOV 15 2021. (15/03965-2, 17/05873-3)

BALERA BRITO, VICTOR GUSTAVO; PATROCINIO, MARIANA SOUSA; LINJARDI SOUSA, MARIA CAROLINA; ALVES BARRETO, AYNA EMANUELLI; TFAILE FRASNELLI, SABRINA CRUZ; LARA, VANESSA SOARES; SANTOS, CARLOS FERREIRA; PENHA OLIVEIRA, SANDRA HELENA. Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production. PLoS One, v. 16, n. 3, MAR 4 2021. (18/23676-3, 17/05873-3, 17/07095-8, 17/02271-2, 15/03965-2)

BRITO, VICTOR GUSTAVO BALERA; PATROCINIO, MARIANA SOUSA; DE SOUSA, MARIA CAROLINA LINJARDI; BARRETO, AYNA EMANUELLI ALVES; FRASNELLI, SABRINA CRUZ TFAILE; LARA, VANESSA SOARES; SANTOS, CARLOS FERREIRA; OLIVEIRA, SANDRA HELENA PENHA. Telmisartan Prevents Alveolar Bone Loss by Decreasing the Expression of Osteoclasts Markers in Hypertensive Rats With Periodontal Disease. FRONTIERS IN PHARMACOLOGY, v. 11, NOV 11 2020. (17/02271-2, 18/23676-3, 17/05873-3, 17/07095-8, 15/03965-2)

BRITO, VICTOR GUSTAVO BALERA; PATROCINIO, MARIANA SOUSA; BARRETO, AYNA EMANUELLI ALVES; FRASNELLI, SABRINA CRUZ TFAILE; LARA, VANESSA SOARES; SANTOS, CARLOS FERREIRA; OLIVEIRA, SANDRA HELENA PENHA. Telmisartan impairs the in vitro osteogenic differentiation of mesenchymal stromal cells from spontaneously hypertensive male rats. European Journal of Pharmacology, v. 912, DEC 5 2021. (17/02271-2, 15/03965-2, 17/05873-3, 18/23676-3)