In vitro reconstituted skin models: development, damage assessment and photoprotective efficacy in the UVA and visible spectral ranges

• Grant number: 17/01272-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2017
• End date: September 30, 2020
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Antonio Claudio Tedesco
• Grantee: Carla Souza
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 19/24530-5 - Bioengineering microvascular network in led light-dermo-epidermal skin model, BE.EP.PD

Abstract

The role of UVB in the development of skin cancer is very well documented. In contrast, the role of UVA still remains unclear and is controversial. This is partly due to the dual role of melanin, as both a photoprotector and a photosensitizer. It has been showed that the visible range can also interfere with the synthesis of melanin and promote skin damages. Therefore, this study aims to evaluate, on a pigmented reconstructed skin model, the effect of UVA and VIS radiation on the skin melanogenesis and, consequently, the role of melanin on DNA damage after radiation, as well as the effectiveness of UVA filters against this damage. Therefore, reconstituted skin models, containing or not human melanocytes will be developed, irradiated by increasing doses of UVA and VIS radiation and evaluated concerning the content and melanin transfer to keratinocytes, melanogenesis gene expression, cell death, oxidative stress, formation of photodimers and photooxidation lesions, as well as the oxidative damage caused to the DNA by flow cytometry, confocal microscopy, PCR and Western blot and the Comet assay. In addition, UVA filters will be evaluated for their ability to protect against the damages caused to DNA. With these results it is expected to establish the production of a pigmented reconstructed skin model and provide additional information on the influence of UVA and VIS radiation on skin melanogenesis and, consequently, the photosensitizing role of melanin and its implications on DNA damage. Finally, it is expected to contribute to the new solar photoprotection strategies being properly proposed.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, CARLA; JAYME, CRISTIANO CERON; REZENDE, NAYARA; TEDESCO, ANTONIO CLAUDIO. Synergistic effect of photobiomodulation and phthalocyanine photosensitizer on fibroblast signaling responses in an in vitro three-dimensional microenvironment. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 222, SEP 2021. (13/50181-1, 18/10237-1, 17/01272-5)

MONICO, DANIELLI AZEVEDO; CALORI, ITALO RODRIGO; SOUZA, CARLA; ESPREAFICO, ENILZA MARIA; BI, HONG; TEDESCO, ANTONIO CLAUDIO. Melanoma spheroid-containing artificial dermis as an alternative approach to in vivo models. Experimental Cell Research, v. 417, n. 1, p. 11-pg., 2022-05-20. (20/04507-6, 13/50181-1, 17/01272-5)

JAYME, CRISTIANO CERON; SOUZA, CARLA; FERNANDES, DANIELA SILVESTRINI; TEDESCO, ANTONIO CLAUDIO. Tailoring the growth and proliferation of human dermal fibroblasts by DNA-based polymer films for skin regeneration. Journal of Biomedical Materials Research Part A, v. 109, n. 11, p. 2381-2391, NOV 2021. (17/01272-5, 18/10237-1, 13/50181-1)

SOUZA, CARLA; MONICO, DANIELLI AZEVEDO; TEDESCO, ANTONIO CLAUDIO. Implications of dichlorofluorescein photoinstability for detection of UVA-induced oxidative stress in fibroblasts and keratinocyte cells. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 19, n. 1, p. 40-48, JAN 1 2020. (13/50181-1, 17/01272-5)

SOUZA, CARLA; PELLOSI, DIOGO SILVA; TEDESCO, ANTONIO CLAUDIO. Prodrugs for targeted cancer therapy. EXPERT REVIEW OF ANTICANCER THERAPY, v. 19, n. 6, p. 483-502, JUN 3 2019. (16/00389-3, 13/50181-1, 17/01272-5)