Effects of radiotherapy in bone-implant interface and on the tissues surrounding

• Grant number: 17/00543-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2017
• Effective date (End): August 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Luana Marotta Reis de Vasconcellos
• Grantee: Rafael Marques Neves
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Radiotherapy is the procedure in which irradiation liberation occurs in specific areas, aiming treatment of tumors. However, acute or chronic damage can develop in the irradiated area and surrounding normal tissues. The objective of this study will be to evaluate the effects of radiotherapy on bone neoformation at the bone-implant interface in the femurs of rats and its influence on the muscular tissue surrounding the irradiated area. In this study 24 rats will receive threaded titanium grade 5 implants in the right femurs. The animals will be divided into 4 groups: a) Implant group (Ip): implant placement surgery (control group - healthy tissue without irradiation); B) Immediate irradiation group (IrI): implant placement surgery followed by irradiation after 1 day; C) Late irradiation group (IrT): implantation surgery and irradiation after 4 weeks; D) Irradiation group (Ir): irradiation and after 4 weeks implant placement surgery. The animals of the groups IrI, IrT and Ir will be submitted to irradiation in a single step in the dose of 30Gy. During the experiment, the activity of contractibility of the muscle surrounding the surgical area will be measured using an electromyograph at two moments. Initially 1 week after implant installation and at the time prior to euthanasia. The animals (n = 6) will be euthanized after 2 weeks of treatment and the pieces will be submitted to computerized microtomography (¼CT), aiming at evaluating bone neoformation at the bone-implant interface and fragments of tissue will be fixed and later separated to histological analysis. Muscle tissue cuts will be stained with Masson trichrome to evaluate the presence or absence of muscle fibrosis. All data will be statistically analyzed, initially through the normality test aiming the application of the appropriate statistical test (p <0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VEGIAN, MARIANA RAQUEL DA CRUZ; COSTA, BRUNO CESAR ALMEIDA; SANTANA-MELO, GABRIELA DE FATIMA; GODOI, FERNANDA HERRERA COSTA; KAMINAGAKURA, ESTELA; TANGO, RUBENS NISIE; DO PRADO, RENATA FALCHETE; DE OLIVEIRA, LUCIANE DIAS; FEDERICO, CLAUDIO ANTONIO; AVELINO, SARAH DE OLIVEIRA MARCO; et al. Systemic and local effects of radiotherapy: an experimental study on implants placed in rats. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 2, p. 785-797, FEB 2020. (17/04389-0, 17/00543-5, 16/19707-5, 15/24986-8)