Respiratory test with endogenous ureia and analyzed by IRMS to detect Helicobacter pylori infection

• Grant number: 17/13021-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2017
• End date: December 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Physics - Nuclear Physics
• Principal Investigator: Vladimir Eliodoro Costa
• Grantee: Ana Beatriz Marques Carlos
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Helicobacter pylori (H. pylori) infection should be present in about half of the world's population and may be associated with peptic ulcers, gastritis, and stomach cancer. Its diagnosis can be made by non-invasive methods such as the stable isotope respiratory test of carbon (13C-UBT). Recently, a new protocol for 13C-UBT was proposed without the need to use 13C-labeled urea in the acid solution and the isotopic ratio of the blow was analyzed by cavity spectroscopy, but this new protocol was not yet verified by the blow analysis by isotopic ratio mass spectrometry (ABCA-IRMS), nor the minimum citric acid required. Thus, the objective of this project is to evaluate the detection of H. pylori infection through 13C-UBT with endogenous urea using the ABCA-IRMS for the analysis of the blow of patients with and without peptic ulcer of the endoscopy sector of Botucatu's Clinical Hospital, and to verify the minimum amount of citric acid, encapsulated and dissolved, in the results of 13C with endogenous urea. First, the protocol of the new 13C-UBT method with endogenous urea in 90 patients infected and not infected by H. pylori with and without ulcer will be evaluated. Subsequently, the same 13C-UBT will be performed again with endogenous urea in another 90 patients, but now with a reduction of half the amount of citric acid and ingested as a capsule. The results of the blow analyzes duplicates will be transformed into means to verify the detection of H. pylori infection; and then compared with the gold standard histopathology to obtain sensitivity and specificity of 13C-UBT with endogenous urea. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARQUES CARLOS, ANA BEATRIZ; DE OLIVEIRA, CASSIO VIEIRA; MARCHESAN RODRIGUES, MARIA APARECIDA; COSTA, VLADIMIR ELIODORO. Is breath testing without C-13-labelled external urea able to detect Helicobacter pylori infection?. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 22, p. 5641-5645, SEP 2019. (17/13021-7)