The use of autologous platelet-rich plasma and heated allogeneic in the treatment of ulcerative keratitis in dogs

• Grant number: 17/13836-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2017
• End date: May 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Cláudia Valéria Seullner Brandão
• Grantee: Annalú Pinton Ferreira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Ulcerative keratitis is a very common ocular condition in veterinary ophthalmology. The cornea, due to its avascular characteristic, presents in its repair mechanism direct relation with inflammatory mediators and growth factors. One of the ways to stimulate corneal repair is through the use of platelet rich plasma (PRP) and in this experiment the heated modality will be tested, since it has the advantage of having less local immunological reaction without damaging the growth factors levels. The objective of this work is to compare the autologous PRP with the heated allogeneic in the treatment of ulcerative keratitis in dogs, as well as their clinical effects. For this, 20 dogs will be used, distributed in two experimental groups (n = 10), presenting corneal ulcer. In the GP group, the dogs will be treated by unheated autologous PRP eye drops and in the GA group, warmed allogeneic PRP eye drops. All dogs will be reassessed: before treatment (M0), three (M3), five (M5), ten (M10), fifteen (M15) and thirty days (M30) after initiation of therapy. The ophthalmologic variables will be evaluated: corneal opacity, vascularization, ocular secretion, chemosis, conjunctival hyperemia, photophobia. These signs will be classified into a score, being (0) absent; (1) discrete; (2) moderate; (3) intense. In addition, the corneal thickness with the pachymeter (Tomey - Japan) will be measured after the instillation of anesthetic eye drops. To evaluate the percentage reduction of corneal ulcer, ImageJ® software, a program developed by the National Institute of Health (NIH), will be used. The hypothesis for this project is that heated allogeneic PRP is a viable adjunctive treatment option for ulcerative keratitis and that it minimizes the local immune response due to its heating. In this way, it can be useful for the implantation of a PRP bank facilitating the dynamics of the service and making possible greater treatment alternatives for patients. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERREIRA, Annalú Pinton. Use of Heated Alogenic and Autologous Platelet-Rich Plasma in the Treatment of Ulcerative Keratitis in Dogs. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.