Comparative stress and immune responses in rainbow trout (Oncurhynvhus mykiss), gilthead seabream (Sparus aurata) after applying two stressors,anoxia and vaccination

• Grant number: 17/22934-6
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: December 11, 2017
• End date: March 09, 2018
• Field of knowledge: Agronomical Sciences - Animal Husbandry
• Principal Investigator: Jaqueline Dalbello Biller Takahashi
• Grantee: Thaís Daltoso da Silva
• Supervisor: Lluis Tort
• Host Institution: Faculdade de Ciências Agrárias e Tecnológicas. Universidade Estadual Paulista (UNESP). Campus de Dracena. Dracena , SP, Brazil
• Institution abroad: Universitat Autònoma de Barcelona (UAB), Spain
• Associated to the scholarship: 16/21443-6 - Immunomodulatory effects of lipopolysaccharide and its evaluation as an alternative to microbiological challenge in tilapia, Oreochromis nilotica, BP.IC

Abstract

The effect of stress on HPI axis activation and gene expression (cytokines and stress genes) will be evaluated in blood, liver and spleen of fish. In the current work, we aim to compare the stress and immune outcomes after stress in rainbow trout (as fresh water model), and gilthead seabream (marine water model). At plasmatic level measurements will include cortisol, glucose and lactate. The gene expression pattern in spleen and liver will be also assessed using gene indicators of stress, immune response and metabolism. The experimental groups will include anoxia, vaccination and an anoxia plus vaccination in order to assess whether additive, synergic or suppressive changes are produced. (AU)