DETECTION AND MOLECULAR DIVERSITY OF Giardia duodenalis IN DOMESTIC ANIMALS AND HUMANS IN THE REGION OF BOTUCATU, SP

• Grant number: 17/14776-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2017
• End date: January 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Regina Kiomi Takahira
• Grantee: Lívia Fagundes Moraes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Giardia, an intestinal parasite of asexual reproduction, is an important etiologic agent of diarrhea in animals and humans, transmitted by orofecal route. Despite the disease caused by this agent, giardiasis, being endemic in the world and representing an important public health problem, little is known about the effective role of animals in this epidemiology. The development of molecular techniques has allowed progress in the identification and classification of these microorganisms. Giardia duodenalis, still considered the only species responsible for human infection, has considerable genetic diversity, consisting of a multi-species complex with eight variants (A-H assemblages). Zoonotic assemblages A and B can also cause disease in dogs and cats. The detection of assemblage A (mainly the AI subclass) has increased in cattle, indicating that this assemblage is more widespread among cattle then initially assumed. Giardia was the most frequent enteroparasite in children aged 0 to 6 years in municipal day care centers and the one with the highest prevalence in the 0 to 4 age group in the city of Botucatu-SP. The objective of the present study is to detect and investigate the molecular diversity of G. duodenalis (SSU-rRNA (ribosomal RNA subunit), gdh (glutamate dehydrogenase) and bg (²-giardin) loci) in domestic animals and humans from Botucatu, SP. In addition, understand the epidemiology of this parasitic disease and evaluate the role of companion and production animals as potential reservoirs of human disease in Botucatu, SP.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAES, LIVIA FAGUNDES; KOZLOWSKI NETO, VITOLDO ANTONIO; DE OLIVEIRA, RAPHAELA MOREIRA; PROVIDELO, GILSON AVELINO; BABBONI, SELENE DANIELA; PINHEIRO FERREIRA, JOAO CARLOS; DOS SANTOS SCHMIDT, ELIZABETH MOREIRA. . Acta Scientiae Veterinariae, v. 47, MAY 7 2019. (17/14776-1)

MORAES, LIVIA FAGUNDES; KOZLOWSKI NETO, VITOLDO ANTONIO; DE OLIVEIRA, RAPHAELA MOREIRA; PROVIDELO, GILSON AVELINO; BABBONI, SELENE DANIELA; PINHEIRO FERREIRA, JOAO CARLOS; DOS SANTOS SCHMIDT, ELIZABETH MOREIRA. Retrospective and Comparative Study of Giardia sp. Prevalence in Dogs, Cats, and Small Ruminants in Endemic Areas in Different Brazilian States. Acta Scientiae Veterinariae, v. 47, p. 10-pg., 2019-05-07. (17/14776-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MORAES, Lívia Fagundes. Molecular diversity of Giardia duodenalis in domestic animals from Botucatu (SP) region. 2021. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.