Effects of temperature and water stress, in climate change scenario, on the fungal community in tropical soil and selection of nitrous oxide producing strains

• Grant number: 17/09000-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2017
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Maria de Lourdes Teixeira de Moraes Polizeli
• Grantee: Tássio Brito de Oliveira
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The global concern about climate change on the planet has been receiving more and more attention. However, the effect of global changes on the soil microbiota, especially fungi, is still incipient and needs to be studied, mainly in tropical areas. In addition, it is urgent to understand how these changes may affect soil metabolism, for example, the selection of species with greater capacity of greenhouse gases production, such as nitrous oxide (N2O). In this context, the project aims to determine how climate change i.e. the elevated temperature and the water stress will affect the community of tropical soil fungi, using a culture-independent method, and the enzymatic activity in soil, as ²-glucosidase, urease, acidic phosphatase, dehydrogenase and cellulase. The experiment will be carried out at the T-FACE (Temperature free-air controlled enhancement) facility installed at the University of São Paulo, Campus of Ribeirão Preto. The isolated fungi under field conditions will be evaluated for their denitrification capacity, by amplification of the P450nor gene, and how much they contribute to N2O emission, when submitted to different nitrogen sources. In addition, it will be possible to evaluate which nitrogen sources contribute most to the emission of this gas. For this, microcosm systems will be used and the application of different nitrogen sources, with and without nitrification inhibitors. The results may help to understand the role of fungi in the flow of N2O in tropical soils and help to propose mitigation strategies or reduction of this gas the emission in a future climate scenario of elevated temperature and water stress.

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SCARCELLA, ANA SILVIA DE ALMEIDA; PASIN, THIAGO MACHADO; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; FERREIRA-NOZAWA, MONICA STROPA; DE FREITAS, EMANUELLE NEIVERTH; VICI, ANA CLAUDIA; BUCKERIDGE, MARCOS SILVEIRA; MICHELIN, MICHELE; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues. ENERGY, v. 226, JUL 1 2021. (14/50884-5, 18/07522-6, 17/09000-4)

ALEXANDER BRUCK, STEFAN; CONTATO, ALEX GRACA; GAMBOA-TRUJILLO, PAUL; DE OLIVEIRA, TASSIO BRITO; CEREIA, MARIANA; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA. Prospection of Psychrotrophic Filamentous Fungi Isolated from the High Andean Paramo Region of Northern Ecuador: Enzymatic Activity and Molecular Identification. MICROORGANISMS, v. 10, n. 2, p. 15-pg., 2022-02-01. (18/07522-6, 08/57908-6, 17/25862-6, 17/09000-4, 14/50884-5)

PASIN, THIAGO MACHADO; DE OLIVEIRA, TASSIO BRITO; DE ALMEIDA SCARCELLA, ANA SILVIA; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA; GUAZZARONI, MARIA-EUGENIA. Perspectives on Expanding the Repertoire of Novel Microbial Chitinases for Biological Control. Journal of Agricultural and Food Chemistry, v. 69, n. 11, p. 3284-3288, MAR 24 2021. (18/07522-6, 17/09000-4)

DE ALMEIDA, PAULA ZAGHETTO; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; SANTOS SALGADO, JOSE CARLOS; PEREZ, MALENA MARTINEZ; GALAN, BEATRIZ; GARCIA, JOSE LUIS; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 beta-glucosidase from Anoxybacillus thermarum. Process Biochemistry, v. 99, p. 1-8, DEC 2020. (18/07522-6, 17/09000-4, 14/50884-5)

CONTATO, ALEX GRACA; VICI, ANA CLAUDIA; PINHEIRO, VANESSA ELISA; DE OLIVEIRA, TASSIO BRITO; DE FREITAS, EMANUELLE NEIVERTH; ARANHA, GUILHERME MAURO; APARECIDO VALVASSORA JUNIOR, ALMIR LUIZ; VARGAS RECHIA, CAREM GLEDES; BUCKERIDGE, MARCOS SILVEIRA; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Comparison of Trichoderma longibrachiatum Xyloglucanase Production Using Tamarind (Tamarindus indica) and Jatoba (Hymenaea courbaril) Seeds: Factorial Design and Immobilization on Ionic Supports. FERMENTATION-BASEL, v. 8, n. 10, p. 12-pg., 2022-10-01. (14/50884-5, 17/25862-6, 18/07522-6, 17/09000-4, 17/23989-9)

DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; PASIN, THIAGO MACHADO; CONTATO, ALEX GRACA; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Cold-Active Lytic Enzymes and Their Applicability in the Biocontrol of Postharvest Fungal Pathogens. Journal of Agricultural and Food Chemistry, v. 68, n. 24, p. 3-pg., 2020-06-17. (17/09000-4)

DE LUCAS, ROSYMAR COUTINHO; DE OLIVEIRA, TASSIO BRITO; LIMA, MATHEUS SANITA; PASIN, THIAGO MACHADO; DE ALMEIDA SCARCELLA, ANA SILVIA; PRADE, ROLF ALEXANDER; SEGATO, FERNANDO; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme (R). BIOMASS CONVERSION AND BIOREFINERY, MAY 2020. (17/09000-4)

DE LUCAS, ROSYMAR COUTINHO; DE OLIVEIRA, TASSIO BRITO; LIMA, MATHEUS SANITA; PASIN, THIAGO MACHADO; DE ALMEIDA SCARCELLA, ANA SILVIA; COSTA RIBEIRO, LILIANE FRAGA; CARVALHO, CAIO; DE LIMA DAMASIO, ANDRE RICARDO; BUCKERIDGE, MARCOS SILVEIRA; PRADE, ROLF ALEXANDER; et al. The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar. RENEWABLE ENERGY, v. 165, n. 1, p. 748-757, MAR 2021. (17/09000-4, 11/02169-7)

DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; CONTATO, ALEX GRACA; PASIN, THIAGO MACHADO; MARTINEZ, CARLOS ALBERTO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Fungal communities differentially respond to warming and drought in tropical grassland soil. Molecular Ecology, v. 29, n. 8, p. 1550-1559, APR 2020. (17/09000-4, 08/58075-8)

DE LUCAS, ROSYMAR COUTINHO; DE OLIVEIRA, TASSIO BRITO; LIMA, MATHEUS SANITA; PASIN, THIAGO MACHADO; DE ALMEIDA SCARCELLA, ANA SILVIA; PRADE, ROLF ALEXANDER; SEGATO, FERNANDO; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme (R). BIOMASS CONVERSION AND BIOREFINERY, v. 12, n. 2, p. 491-499, FEB 2022. (17/09000-4)

CONTATO, ALEX GRACA; DE OLIVEIRA, TASSIO BRITO; ARANHA, GUILHERME MAURO; DE FREITAS, EMANUELLE NEIVERTH; VICI, ANA CLAUDIA; VIEIRA NOGUEIRA, KAROLINE MARIA; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; BUCKERIDGE, MARCOS SILVEIRA; SILVA, ROBERTO NASCIMENTO; et al. Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse. MICROORGANISMS, v. 9, n. 3, MAR 2021. (14/50884-5, 18/25898-3, 17/23989-9, 18/07522-6, 17/09000-4, 17/25862-6, 08/57908-6)

DE LUCAS, ROSYMAR COUTINHO; DE OLIVEIRA, TASSIO BRITO; LIMA, MATHEUS SANITA; PASIN, THIAGO MACHADO; DE ALMEIDA SCARCELLA, ANA SILVIA; COSTA RIBEIRO, LILIANE FRAGA; CARVALHO, CAIO; DE LIMA DAMASIO, ANDRE RICARDO; BUCKERIDGE, MARCOS SILVEIRA; PRADE, ROLF ALEXANDER; et al. The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar. RENEWABLE ENERGY, v. 165, p. 10-pg., 2021-03-01. (17/09000-4, 11/02169-7)

MELO, WEILAN GOMES DA PAIXAO; DE OLIVEIRA, TASSIO BRITO; ARCURI, SILVIO LOVATO; DE MORAIS, PAULA BENEVIDES; PAGNOCCA, FERNANDO CARLOS. Yeasts in the nests of the leaf-cutter ant Acromyrmex balzani in a Savanna biome: exploitation of community and metabolic diversity. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. 114, n. 6, MAR 2021. (12/14594-7, 15/01001-6, 17/09000-4)

DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; PASIN, THIAGO MACHADO; MARTINEZ, CARLOS ALBERTO; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA. Perspectives on Exploring Denitrifying Fungi as a Model To Evaluate Nitrous Oxide Production and Reduce Emissions from Agricultural Soils. Journal of Agricultural and Food Chemistry, v. 67, n. 44, p. 2-pg., 2019-11-06. (08/58075-8, 17/09000-4)

PASIN, THIAGO MACHADO; SANTOS SALGADO, JOSE CARLOS; DE ALMEIDA SCARCELLA, ANA SILVIA; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; CEREIA, MARIANA; ROSA, JOSE CESAR; WARD, RICHARD JOHN; BUCKERIDGE, MARCOS SILVEIRA; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. A Halotolerant Endo-1,4-beta-Xylanase fromAspergillus clavatuswith Potential Application for Agroindustrial Residues Saccharification. Applied Biochemistry and Biotechnology, v. 191, n. 3, p. 1111-1126, JUL 2020. (17/09000-4, 14/50884-5)

DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; CONTATO, ALEX GRACA; PASIN, THIAGO MACHADO; MARTINEZ, CARLOS ALBERTO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Effects of multiple climate change factors on exoenzyme activities and CO2 efflux in a tropical grassland. SOIL BIOLOGY & BIOCHEMISTRY, v. 148, SEP 2020. (17/09000-4, 08/58075-8)