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Proteomics applied to the research of renal biomarkers in hypertensive and type 1 diabetic rats

Grant number: 17/16231-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2018
Effective date (End): December 31, 2018
Field of knowledge:Biological Sciences - Biology
Principal Investigator:Pedro de Magalhães Padilha
Grantee:Janaína Macedo da Silva
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The world scenario is alarming about the incidence of diabetes mellitus, it is currently estimated that there are 382 million people worldwide with the pathology, and that by 2035 this number should reach 471 million. Diabetes mellitus type1 (DM1) is a chronic endocrine-metabolic disorder characterized by total or partial destruction the beta cells of the islets of pancreatic Langerhans, with an incidence of approximately 0.5 new cases per year for every 100.000 inhabitants, affecting mainly adolescents. Among the complications triggered by DM1 there are hypertensions, both of which are classified as chronic non-transmissible diseases (DCNT), causing a considerable loss of quality of life and high drug costs. Considering the problems involved, this work aims to optimize strategies to fractionate proteome and to identify proteins differently expressed in the renal tissue of hypertensive rats and affected with DM1. Thus, the challenge is to prepare the sample groups appropriately, as well as to correctly manipulate the biological samples, in order to obtain scientifically satisfactory results at the end of the project. For this purpose, 20 rats (± 250g) distributed in the experimental groups (n = 5) will be used: C = normal control; CD = diabetic control, H = hypertensive control (SHR) and DH = diabetic SHR. DM1 will be induced with streptozotocin (45 mg kg-1 body, i.p.). After the experimental period (37 days) the animals will be anesthetized, euthanized and samples of renal tissue will be collected. The next steps will be the following: optimization of protein fraction extraction and precipitation strategies, proteome fractionation by two-dimensional electrophoresis (2D-PAGE) and identification of differentially expressed protein spots in relation to experimental groups by analysis of 2D PAGE gels. The results obtained in the work proposal will contribute to the characterization by mass spectrometry (ESI-MS / MS) of biomarkers that help in understanding the correlation between the higher incidence of hypertension in individuals affected with type 1 diabetes. (AU)