Identification of candidate interactors and global response pathways activated by Sporisorium scitamineum effectors

• Grant number: 17/25477-5
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): March 01, 2018
• Effective date (End): August 31, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal researcher: Claudia Barros Monteiro Vitorello
• Grantee: Natália de Sousa Teixeira e Silva
• Supervisor abroad: Walter Gassmann
• Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Research place: University of Missouri, Columbia (UM), United States
• Associated to the scholarship: 16/16376-8 - Functional analysis of candidate effector proteins during Sporisorium scitamineum x sugarcane interaction, BP.DR

Abstract

Sugarcane smut is a worldwide distributed disease recognized as one of the major threats for sugarcane production. The disease is caused by the biotrophic basidiomycete fungus Sporisorium scitamineum. Cane-smut interaction has been studied by our research group during the past few years in their various aspects, considering both fungal pathogenesis and global host responses. The aim of this project is to study the interactome of candidate effector proteins of this pathosystem. Effectors are essential to modulate host metabolism in favor of pathogen colonization. An overview of effector interactions may assist the recognition of candidate and pathways relevant for genetic breeding programs. Potential effectors were selected based on S. scitamineum complete genome sequence and differential expression data obtained by RNAseq. The interactome of candidates will be analyzed by co-immunoprecipitation assay, BiFC technique and through a protoplast screening platform to capture protein complexes and evaluate the pathways they influence. These results will enable future researches considering virulence level of different isolates and also help decision making in plant breeding programs for more resistant varieties. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TEIXEIRA-SILVA, NATALIA SOUSA; CARVALHO SCHAKER, PATRICIA DAYANE; SILVA RODY, HUGO VIANNA; MAIA, THIAGO; GARNER, CHRISTOPHER M.; GASSMANN, WALTER; MONTEIRO-VITORELLO, CLAUDIA BARROS. Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors. JOURNAL OF FUNGI, v. 6, n. 4, DEC 2020. (16/04429-0, 17/13268-2, 13/25599-2, 18/04555-0, 17/25477-5, 16/17545-8, 16/16376-8)