MITOCHONDRIAL BIOGENESIS IN SKELETAL MUSCLE: EFFECT ON OXIDATIVE STRESS

• Grant number: 17/20387-8
• Support Opportunities: Scholarships abroad - Research
• Effective date (Start): July 26, 2018
• Effective date (End): July 25, 2019
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Anderson Saranz Zago
• Grantee: Anderson Saranz Zago
• Host Investigator: Joe Quadrilatero
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
• Research place: University of Waterloo, Canada

Abstract

Mitochondria are the main source of free radicals and antioxidants, and any change in it biogenesis may favor the production of reactive oxygen species (ROS), triggering a series of factors that compromise blood pressure control. Several studies indicate that the increase of oxidative stress is associated with a reduction of functional mitochondria integrity, activation of some pro-inflammatory transcription pathways and activation of apoptosis induction factors. On the other hand, the increase in mitochondrial biogenesis is strongly associated with the reduction of such factors in the skeletal muscle. In this way the purpose of this project is to analyze the effect of increase or decrease mitochondrial biogenesis on the apoptosis, oxidative stress and inflammation parameters in skeletal muscle cell culture, and the effect of electrical stimulation on such relationships. The increase in mitochondrial biogenesis will be performed by nitric oxide supplementation and electrical stimulation, which has similar effects to physical exercise practice in vivo. The decrease in mitochondrial biogenesis will be performed by glucose supplementation and interference RNA insertion. Expression of genes involved in mitochondrial biogenesis and fragmentation will be analyzed by RT-PCR and Spectromax Plus Spectrophotometer respectively. The mitochondrial content will be determined by fluorescent probe Mito Tracker Green and analyzed by Flow Cytometer. The content of the proteins will be determined by Western Blotting and the content of hydrogen peroxide and ROS by fluorescence (Spectra max). Furthermore, inflammatory parameters and enzymatic activity will be performed by ELISA through commercial kits and fluorescence respectively. The data will be analyzed by ANOVA one-way and, for all cases a p <0.05 will be considered for significance.