Study of crude glycerol addition to Rhodosporidium toruloides culture in hemicellulosic hydrolysate

• Grant number: 17/25644-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2018
• End date: November 30, 2018
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal Investigator: Everson Alves Miranda
• Grantee: Alex Ikeda Francisco
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales, AP.BIOEN.TEM

Abstract

So far, we have studied at a good level of depth the adaptation of yeast to hemicellulosic hydrolysate, the effect of the inhibitors present in this hydrolysate, supplementation of the culture medium with salts, and the separation of the cells from the fermented broth by flotation. All these cultivation studies were carried out in agitated Erlenmeyer flasks. An experiment in a single condition of cultivation in a bioreactor with the addition of glycerol allowed for values of lipid content much higher than those achieved in flasks, from 34 to 56%. Thus, this IC research aims the study the effect of crude glycerol addition to the hemicellulosic hydrolysate on growth kinetics and oil production. This study will be the basis for the study in bioreactor to be performed by a postdoctoral researcher. (AU)