Occurrence of Coxiella burnetii in free living marsh deer (Blastocerus dichotomus) in Brazil

• Grant number: 17/23831-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2018
• End date: January 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcos Rogério André
• Grantee: Júlia Banhareli Tasso
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Coxiella burnetii, the etiological agent of Q fever, is a Gram-negative, pleomorphic and obrigate intracellular bacilocco, that belongs to order Legionalles and Family Coxiellaceae. The Q Fever, which is considered a zoonosis, is mainly transmitted by hematophagus arthropods (such as ticks) or by aerosols. Domestic and wild ruminants have an extremely importance in the distribution of this agent, involving economic aspects and Public Health. In this sense, the present study aimed to verify the ocurrence, by molecular methods, of C. burnetii in blood samples from free-ranging Brazilian marsh deer (Blastocerus dichotomus) in Brazil. For this purpose, 143 blood samples will be submitted to nested PCR (nPCR) technique based in the repetitive insert element IS1111 of the heat shock protein (htpAB) gene. Thereafter, nPCR amplicons will be purified and sequenced and their identity will be validate and compared to sequences previously deposited in Genbank. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDRE, MARCOS ROGERIO; CALCHI, ANA CLAUDIA; HERRERA, HEITOR MIRAGLIA; DE SOUZA ZANATTO, DIEGO CARLOS; LE SENECHAL HORTA, BRUNA DE CARVALHO; TASSO, JULIA BANHARELI; DE SOUZA RAMOS, INALDA ANGELICA; CALIFRE DE MELLO, VICTORIA VALENTE; MACHADO, ROSANGELA ZACARIAS. The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal. VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, v. 21, JUL 2020. (18/13838-6, 17/23831-6, 17/23327-6, 18/02753-0)

DE SOUZA ZANATTO, DIEGO CARLOS; BARBANTI DUARTE, JOSE MAURICIO; LABRUNA, MARCELO BAHIA; TASSO, JULIA BANHARELI; CALCHI, ANA CLAUDIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Evidence of exposure to Coxiella burnetii in neotropical free-living cervids in South America. Acta Tropica, v. 197, SEP 2019. (17/23831-6)