Functional characterization and evaluation of the antigenicity and immunogenicity of recombinant proteins of Mycoplasma agalactiae

• Grant number: 16/23306-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2018
• End date: November 30, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Jorge Timenetsky
• Grantee: Maysa Santos Barbosa
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 19/03425-9 - Antigenic characterization of the membrane proteins of Mycoplasma agalactiae and them role in virulence and pathogenicity in vitro, BE.EP.DR

Abstract

Contagious Agalactia (CA) affects small ruminants and is classified by the World Organisation for Animal Health (OIE) as a notifiable disease. CA has significant economic impact on the production of sheep and goats. It causes reduction or suppression of milk production, Fever, Septicemia, Mastitis, abortion and Severe Arthritis, and may cause death quickly. CA is a multi etiologic syndrome, in which three microorganisms were identified as the cause of the disease: Mycoplasma agalactiae, the most frequent pathogen; M. mycoides subsp. capri and M. capricolum subsp. capricolum. Despite the progress in diagnostic assays, therapeutics and prevention methods, current strategies are still inefficient in controlling the AC. Thus, the generation of new alternatives of diagnostic and protection against infections by M. agalactiae are required. Thus, the aim of this project is to functionally characterize and evaluate the antigenicity and immunogenicity, in sheep, of four recombinant M. agalactiae proteins previously identified as antigens in our laboratory. It is expected that the results of this study will lead to the development of an immunodiagnostic assay and an immunotherapy alternative that are more efficient than those available in Europe and can be used in this country. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARBOSA, MAYSA SANTOS; MARQUES, LUCAS MIRANDA; TIMENETSKY, JORGE; ROSENGARTEN, RENATE; SPERGSER, JOACHIM; CHOPRA-DEWASTHALY, ROHINI. Host cell interactions of novel antigenic membrane proteins of Mycoplasma agalactiae. BMC Microbiology, v. 22, n. 1, p. 13-pg., 2022-04-08. (17/25686-3, 19/03425-9, 16/23306-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BARBOSA, Maysa Santos. Surface proteins from Mycoplasma agalactiae act as adhesins, interact with host proteins and activate DNA damage pathways.. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.