Adipose-specific knockout of acyl-CoA synthase 4 (ACSL4) and the obesity metabolism control

• Grant number: 17/21676-3
• Support Opportunities: Scholarships abroad - Research
• Start date: August 01, 2018
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Ellen Cristini de Freitas
• Grantee: Ellen Cristini de Freitas
• Host Investigator: Andrew Greenberg
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Tufts University, United States

Abstract

The five isoforms of the long-chain acyl-CoA synthases (ACSL) are associated with different roles in the regulation of fatty acid metabolism, promotion of cell proliferation and apoptosis, and development of diseases. The effects of ACSL on fatty acid metabolism are well documented; however, their roles in controlling obesity metabolism must be better investigated. Therefore, this project will verify the effects of adipocyte-specific ACSL4 knockout on metabolic parameters, body composition, insulin sensitivity, plasma levels of insulin, b-hydroxybutyrate, triglyceride, and cholesterol, adipocyte lipolysis, number and size of adipocytes, and genes related to energy expenditure, inflammation, lipid metabolism and white adipose tissue differentiation in diet-induced obese mice. Mice lacking ACSL4 in adipocyte tissue and control mice will be caged and fed a low-fat (LFD) or high-fat diet (HFD) for 12 weeks. Data will be compared using an unpaired two-tailed Student's t-test, and significance will be set a P < 0.05.Keywords: ACSL4; white adipose tissue; lipolysis; oxygen consumption; molecular biology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACDONALD, TARA L.; PATTAMAPRAPANONT, PATTARAWAN; PATHAK, PRERANA; FERNANDEZ, NATALIE; FREITAS, ELLEN C.; HAFIDA, SAMAR; MITRI, JOANNA; BRITTON, STEVEN L.; KOCH, LAUREN G.; LESSARD, SARAH J.. Hyperglycaemia is associated with impaired muscle signalling and aerobic adaptation to exercise. NATURE METABOLISM, v. 2, n. 9, p. 902+, SEP 2020. (17/21676-3)