Functional studies of the Xanthomonas citri pv. citri type 6 secretion system: secreted proteins, recruitment and regulation

• Grant number: 17/02318-9
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2018
• End date: October 31, 2022
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Cristina Elisa Alvarez Martinez
• Grantee: Lucas de Moraes Ceseti
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 20/11140-1 - Characterisation of candidate effectors of the Xanthomonas citri pv. citri type 6 secretion system using the Dictyostelium discoideum model, BE.EP.DD

Abstract

Type VI Secretion Systems (T6SS) are specialized machines that deliver effector proteins directly into prokaryotic and/or eukaryotic target cells. T6SS are widespread in Proteobacteria, particularly within the Gamma subdivision. Several members of this group are involved in interspecific competition and play a fundamental role in bacterial fitness in their ecological niche. The genome of the phytopathogen Xanthomonas citri pv citri encodes a complete T6SS, which is required for bacterial survival against predation by Dictyostelium discoideum, a soil-living amoeba that likely cohabits with Xac in the environment. This project aims to identify proteins secreted by the Xac T6SS, as well as the mechanisms of their specific recruitment for secretion. Also, we propose to characterize the posttranslational control of T6SS activity. Data obtained in this work will help to elucidate the mechanisms of T6SS function in Xac environmental fitness, a key step of the citrus canker disease cycle. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, LIDIA DOS PASSOS; PEREIRA, JULIANA BIAR; FASABI FLORES, ANTHONY JHOAO; RODRIGUES LORENZETTI, ALAN PERICLES; BOECHAT, ANA LAURA; PEREDA, MARIA CLAUDIA; GUALTIERI, SOPHIA; DO PRADO, DANIELE FERREIRA; ROCHA, DIEGO; CESETI, LUCAS DE MORAES; et al. An Extracytoplasmic Function Sigma Factor Required for Full Virulence in Xanthomonas citri pv. citri. Journal of Bacteriology, v. 204, n. 5, p. 19-pg., 2022-04-21. (18/01852-4, 17/03052-2, 19/03406-4, 17/02318-9)

BAYER-SANTOS, ETHEL; CESETI, LUCAS DE MORAES; FARAH, CHUCK SHAKER; ALVAREZ-MARTINEZ, CRISTINA ELISA. Distribution, Function and Regulation of Type 6 Secretion Systems of Xanthomonadales. FRONTIERS IN MICROBIOLOGY, v. 10, JUL 17 2019. (18/04553-8, 17/17303-7, 17/02178-2, 18/01852-4, 17/02318-9)

CESETI, LUCAS M.; DE SANTANA, ELIANE S.; RATAGAMI, CAMILA Y.; BARREIROS, YASMIN; LIMA, LIDIA DOS PASSOS; DUNGER, GERMAN; FARAH, CHUCK S.; ALVAREZ-MARTINEZ, CRISTINA E.. The Xanthomonas citri pv. citri Type VI Secretion System is Induced During Epiphytic Colonization of Citrus. Current Microbiology, v. 76, n. 10, p. 1105-1111, OCT 2019. (11/22571-4, 16/08400-6, 14/19720-6, 17/02318-9, 18/01852-4, 14/25261-4, 11/07777-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CESETI, Lucas de Moraes. Functional studies of the Xanthomonas citri subsp. citri type VI secretion system: secreted proteins, recruitment and regulation. 2023. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.