Evaluation of the cytogenetic indicator of the bleeding epithelium in newborn the term

• Grant number: 17/07861-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2018
• End date: December 31, 2018
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Marisa Afonso de Andrade Brunherotti
• Grantee: Aline Cardoso Brito Pinho
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Infant mortality, especially in the first days of life, is a concern of the world's public health. However, newborn survival has increased among children up to five years, as a result of government mobilization and local initiatives for interventions and research for maternal and child care. Negative influence of the environment on gestation, such as maternal exposure to certain viruses, drugs, tobacco habit, alcoholism among others may contribute in addition to infant mortality with fetal malformations and neonatal complications. Maternal exposure to environmental, occupational, and lifestyle toxic factors related to the increased frequency of micronuclei, which may recognize cellular DNA damage, could contribute to the future development of newborns. The investigation. The test that has been used as a minimally invasive method for evaluation of DNA damage by genotoxic agents in adults and children is the micronucleus test in exfoliated cells of the oral cavity. The investigation of the micronucleus in the newborn is very recent and the literature is scarce, the investigation of the micronucleus in the newborn group could be of great value for the identification of risks and orientation of the best therapeutic approach in order to prevent sequelae in this population. The objective of the present study is to evaluate the presence and frequency of the micronucleus marker in exfoliated cells of the buccal mucosa of term newborns associated with maternal prenatal factors. The study will be analytical, observational and longitudinal in the public tertiary-level hospital. Data will be from term newborns accompanied by birth to discharge. The data will be obtained initially from medical records and after collecting the epithelial mucosal smear from the mouth (micronucleus markers) every two days from birth to hospital discharge. For the comparison of the variables, the paired "t" test will be used to analyze the parametric data and the Mann-Whitney test for the non-parametric data, adopting a significance of 5%. (AU)