Study of the effects of the photobiostimulation on the Schwann cell mitochondrial metabolism in Type 1 Diabetes Mellitus

• Grant number: 17/25554-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): May 01, 2018
• Status: Discontinued
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal researcher: Marucia Chacur
• Grantee: Igor Rafael Correia Rocha
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 20/15777-4 - Inhibition of Neural Cells by DREADDs in the Nervous System, BE.EP.DR

Abstract

Primary cell damage from Schwann cells in hyperglycemia is largely neglected in terms of Diabetic Peripheral Neuropathy. This situation results from the historical attention given to micro and macrovascular diseases from Diabetes. In contrast, the mechanisms by which low level laser therapy generates its promising effects is still the field of extensive research. It is proposed here, through the study of the interaction between photobiostimulation and mitochondrial energy metabolism in Type 1 Diabetes Mellitus, to generate data that can elucidate the mechanisms involved in the pathophysiology of diabetic peripheral neuropathy as well as try to clarify the mechanisms that measure the functional aspects between the interactions of light (laser) and the nervous tissue, especially in the process of repairing peripheral nerve fibers. In terms of mitochondrial functioning, the study aspects will be: analysis of mitochondrial membrane potential, oxygen consumption (mitochondrial respiration) analysis and also the analyzes of reactive oxygen species generation by this organelle. On the other hand we will analyze mitochondrial dynamism through the analyzes of the proteins related to mitochondrial fusion and fission processes, mitofusin -2 and DRP-1 respectively. In addition, as regards Schwann cells, proteins that are closely related to the myelinization process of peripheral nerve fibers, for example, Myelin Basic Protein (MBP), Myelin Regulatory Factor (MRF) the Myelin Expression Factor 2 (MEF2) will be analyzed as well. The understanding of the aforementioned mechanisms can elucidate the means by which the deterioration of the mitochondrial functioning of the Schwann cells in hyperglycemic medium can influence the noxious process caused to the axons of the peripheral nerves and how the participation of the low level laser can mediate the process of reversion of the noxious damage. These results may be influential for the development of new forms of therapies aimed at restoring peripheral nerve fibers in Diabetes Mellitus, bringing a new perspective on quality of life for those suffering from Diabetic Peripheral Neuropathy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREIA ROCHA, IGOR RAFAEL; CHACUR, MARUCIA. Modulatory effects of photobiomodulation in the anterior cingulate cortex of diabetic rats. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, v. 20, n. 6, p. 781-790, JUN 2021. (17/25554-0)