Lignocellulosic biomass of Panicum maximum Jacq. forage grass produced in simulated future climate conditions: potential source of fermentable sugars for bioenergy

• Grant number: 17/23989-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2018
• End date: November 30, 2021
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Maria de Lourdes Teixeira de Moraes Polizeli
• Grantee: Emanuelle Neiverth de Freitas
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 19/07141-5 - Formulation of an optimized synergistic enzyme cocktail with accessory enzymes to enhances the hydrolytic performance of Panicum maximum Jacq., BE.EP.DR

Abstract

The depletion of fossil fuels and the pollution generated by them, such as the emission of greenhouse gases, has stimulated the search for alternative and renewable sources of energy. Biomass produced by fast-growing grasses such as Panicum genus, commonly used as fodder, can be important sources of clean energy production, but it is necessary to estimate their biomass potential production in simulated future climate situations. Lignocellulosic biomass is mainly composed of cellulose, hemicellulose and lignin, and therefore, it is necessary the joint action of many enzymes for fermentable sugars production. However, lignin presents as a barrier to the action of cellulolytic enzymes, making necessary a pretreatment process in order to increase the accessibility of the enzymes on the cellulosic portion of the biomass. Many pretreatment methods are available, however, biological pretreatment seems to be the most promising, since it does not generate fermentation inhibitory compounds and is considered an eco-friendly process. The present work aims to evaluate the potential of the Panicum maximum Jacq. Mombaça variety as an alternative source for bioenergy, after being submitted to high temperature treatment (+ 2 ° C) and higher CO2 concentrations (600ppm), simulating future climatic conditions. It will be studied different lignocellulolytic enzymes from fungi, aiming the development of an enzymatic consortium with potential to be applied in industries or in biological pre-treatments, in order to increase the hydrolysis yield. To evaluate the morphological and chemical characteristics of the biomass before and after the pretreatment, an Infrared Spectrophotometer with Fourrier Transform (FTIR), Scanning Electron Microscope, X-Ray Diffraction and chemical composition analysis will be used. Depending on the project approval, an extension of the research plan can be developed under the supervision of Prof. Dr. Leonardo Gomez at York University, aiming to increase the efficiency of the process by digestion and cell wall studies.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE FREITAS, EMANUELLE N.; KHATRI, VINAY; CONTIN, DANIELE R.; DE OLIVEIRA, TASSIO B.; CONTATO, ALEX G.; PERALTA, ROSANE M.; DOS SANTOS, WANDERLEY D.; MARTINEZ, CARLOS A.; SADDLER, JACK N.; POLIZELI, MARIA DE LOURDES T. M.. limate change affects cell-wall structure and hydrolytic performance of a perennial grass as an energy cro. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. 16, n. 2, NOV 2021. (08/58075-8, 08/57908-6, 17/23989-9, 19/07141-5, 18/07522-6)

DE FREITAS, EMANUELLE N.; KHATRI, VINAY; WU, JIE; TAKADA, MASATSUGU; SCARCELLA, ANA SILVIA DE A.; MARTINEZ, CARLOS ALBERTO; SADDLER, JACK N.; POLIZELI, MARIA DE LOURDES T. M.. Structural and compositional changes induced by hydrothermal and organosolv pretreatments impacts enzymatic hydrolysis of a tropical forage grass grown under future climate conditions. INDUSTRIAL CROPS AND PRODUCTS, v. 171, NOV 1 2021. (17/23989-9, 18/07522-6, 19/07141-5, 08/57908-6, 08/58075-8)

DE FREITAS, EMANUELLE NEIVERTH; ALNOCH, ROBSON CARLOS; CONTATO, ALEX GRACA; NOGUEIRA, V, KAROLINE MARIA; CREVELIN, EDUARDO JOSE; BERALDO DE MORAES, LUIZ ALBERTO; SILVA, ROBERTO NASCIMENTO; MARTINEZ, CARLOS ALBERTO; POLIZELI, MARIA DE LOURDES T. M.. Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 17, SEP 2021. (18/07522-6, 19/07141-5, 08/57908-6, 17/23989-9, 08/58075-8, 20/00081-4)

CONTATO, ALEX GRACA; VICI, ANA CLAUDIA; PINHEIRO, VANESSA ELISA; DE OLIVEIRA, TASSIO BRITO; DE FREITAS, EMANUELLE NEIVERTH; ARANHA, GUILHERME MAURO; APARECIDO VALVASSORA JUNIOR, ALMIR LUIZ; VARGAS RECHIA, CAREM GLEDES; BUCKERIDGE, MARCOS SILVEIRA; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Comparison of Trichoderma longibrachiatum Xyloglucanase Production Using Tamarind (Tamarindus indica) and Jatoba (Hymenaea courbaril) Seeds: Factorial Design and Immobilization on Ionic Supports. FERMENTATION-BASEL, v. 8, n. 10, p. 12-pg., 2022-10-01. (14/50884-5, 17/25862-6, 18/07522-6, 17/09000-4, 17/23989-9)

CONTATO, ALEX GRACA; DE OLIVEIRA, TASSIO BRITO; ARANHA, GUILHERME MAURO; DE FREITAS, EMANUELLE NEIVERTH; VICI, ANA CLAUDIA; VIEIRA NOGUEIRA, KAROLINE MARIA; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; BUCKERIDGE, MARCOS SILVEIRA; SILVA, ROBERTO NASCIMENTO; et al. Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse. MICROORGANISMS, v. 9, n. 3, MAR 2021. (14/50884-5, 18/25898-3, 17/23989-9, 18/07522-6, 17/09000-4, 17/25862-6, 08/57908-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FREITAS, Emanuelle Neiverth de. Lignocellulosic biomass of Panicum Maximum Jacq forage grass produced in simulated future climate conditions: a potential source of fermentable sugars for bioenergy. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.