Evaluation of estradiol effects on lung of brain dead female rats after ex vivo perfusion

• Grant number: 18/07289-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): October 01, 2018
• Effective date (End): September 30, 2019
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Ana Cristina Breithaupt Faloppa
• Grantee: Fernanda Yamamoto Ricardo da Silva
• Supervisor: Hendrik Gerrit Derk Leuvenink
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Research place: University Medical Center Groningen (UMCG), Netherlands
• Associated to the scholarship: 16/03692-9 - Evaluation of the estradiol treatment on pulmonary inflammaton on female rats subject to brain death, BP.DR

Abstract

Lung transplant, which often comes from brain dead (BD) donors, is the treatment of choice for severe lung disease. However the low numbers of viable lungs for transplantation impacts on the patient treatment capacity. Clinical and experimental studies highlight the impact of BD on lung viability for transplant, starting with the hemodynamic instability and inflammatory and immunological alterations. In order to improve organ quality, technics such as ex vivo lung perfusion has been used. At the same time, studies point to worse prognosis in lung transplants from female donors to male recipients. Also, previous results from our laboratory, using an experimental model of BD in rats, show a higher lung inflammation in female rats, associated with the acute reduction of female sex hormones. These findings together with clinical and experimental studies showing protective effects of estradiol on the immune system, lung inflammation and release of pro-inflammatory mediators, reinforce the importance of studying estradiol effects on the control of lung inflammatory response in female rats submitted to brain death. Therefore, this study intends to evaluate the effect of ex vivo lung perfusion with estradiol on BD female lungs. In that sense, the association between the ex vivo lung perfusion with a possible protective effect of estradiol could become a therapeutic alternative to mitigate the deleterious effects of brain death.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDA YAMAMOTO RICARDO-DA-SILVA; ROBERTO ARMSTRONG-JR; MARINA VIDAL-DOS-SANTOS; CRISTIANO DE JESUS CORREIA; RAPHAEL DOS SANTOS COUTINHO E SILVA; LUCAS FERREIRA DA ANUNCIAÇÃO; LUIZ FELIPE PINHO MOREIRA; HENRI GERRIT DERK LEUVENINK; ANA CRISTINA BREITHAUPT-FALOPPA. Long–term lung inflammation is reduced by estradiol treatment in brain dead female rats. Clinics, v. 76, 2021-08-16. (16/03692-9, 17/11798-4, 18/07289-0)

RICARDO-DA-SILVA, FERNANDA YAMAMOTO; ARMSTRONG JR, ROBERTO; VIDAL-DOS-SANTOS, MARINA; CORREIA, CRISTIANO DE JESUS; COUTINHO E SILVA, RAPHAEL DOS SANTOS; DA ANUNCIACAO, LUCAS FERREIRA; PINHO MOREIRA, LUIZ FELIPE; DERK LEUVENINK, HENDRIK GERRIT; BREITHAUPT-FALOPPA, ANA CRISTINA. 17 beta-Estradiol Treatment Protects Lungs Against Brain Death Effects in Female Rat Donor. TRANSPLANTATION, v. 105, n. 4, p. 775-784, APR 2021. (17/11798-4, 18/07289-0, 16/03692-9)

RICARDO-DA-SILVA, FERNANDA YAMAMOTO; ARMSTRONG-JR, ROBERTO; VIDAL-DOS-SANTOS, MARINA; CORREIA, CRISTIANO DE JESUS; SILVA, RAPHAEL DOS SANTOS COUTINHO E; ANUNCIACAO, LUCAS FERREIRA DA; MOREIRA, LUIZ FELIPE PINHO; LEUVENINK, HENRI GERRIT DERK; BREITHAUPT-FALOPPA, ANA CRISTINA. Long-term lung inflammation is reduced by estradiol treatment in brain dead female rats. Clinics, v. 76, 2021. (16/03692-9, 17/11798-4, 18/07289-0)