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Effect of physical exercise on induced nociception in the Parkinson's Disease model in rats

Grant number: 17/26821-1
Support type:Scholarships in Brazil - Master
Effective date (Start): June 01, 2018
Effective date (End): August 31, 2020
Field of knowledge:Biological Sciences - Physiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Marucia Chacur
Grantee:Karina Henrique Binda
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):18/25204-1 - Role of opioids, cannabinoids, dopamine and neuroinflammation systems on pain threshold modulated by physical exercise in a rat Parkinson Disease model using a radioactive labeled substances, BE.EP.MS

Abstract

Parkinson's disease (PD) is the second most common neurodegenerative disease among elderly people, causing a large socioeconomic impact with increasing life expectancy of worldwide. In addition to motor symptoms, there is a high prevalence of pain symptoms which can be responsible for worsening patient's quality of life. Physical exercise, in turn, has already been described for its beneficial effects on PD with decreased neuronal loss in animal models, improved quality of life and pain symptoms of patients. The aim of this study will be investigate the possible mechanisms involved in the improvement of chronic pain reported by patients diagnosed with a PD after physical exercise protocols in a model of unilateral Parkinson's disease induced by 6-hydroxydopamine (6-OHDA), a non-pharmacological therapeutic intervention widely used. Thus, we will evaluate the nociceptive (mechanical hyperalgesia) and motor (cylinder test) behaviors, and the expression of opioid (mi, delta and Kapa) and cannabinoid (CB1 and CB2) receptors in regions of the nervous system involved with neural circuit of pain, being: periaqueductal gray (PAG), thalamus and anterior cingulate cortex (ACC). For receptors expression analysis immunohistochemistry and Western blot techniques will be performed. The data will be collected from male rats submitted to PD like model, and to treadmill exercise protocol. Exercise will be performed 3 times per week for 40 minutes during 5 weeks at 10 m/min. Motor behavior will be investigated at 3 timepoints, being: basal (before PD model induction), at 7 and 51 days after PD model induction. However, nociceptive behavior will be evaluated at 6 different moments, being: baseline (before induction of the model), 7, 14, 23, 37 and 51 days after the induction of the PD model. At the end of protocol experiments, animals will be euthanized to perform the techniques described above. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BINDA, K. H.; LILLETHORUP, T. P.; REAL, C. C.; BAERENTZEN, S. L.; NIELSEN, M. N.; ORLOWSKI, D.; BROOKS, D. J.; CHACUR, M.; LANDAU, A. M. Exercise protects synaptic density in a rat model of Parkinson's disease. Experimental Neurology, v. 342, AUG 2021. Web of Science Citations: 0.
BINDA, K. H.; REAL, C. C.; FERREIRA, A. F. F.; BRITTO, L. R.; CHACUR, M. Antinociceptive effects of treadmill exercise in a rat model of Parkinson's disease: The role of cannabinoid and opioid receptors. Brain Research, v. 1727, JAN 15 2020. Web of Science Citations: 0.

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