FRACTIONATION AND ISOLATION OF COMPOUNDS FROM ETHANOLIC EXTRACTS OF Schinus terebinthifolius Raddi BY COUNTER CURRENT CHROMATOGRAPHY

• Grant number: 18/11823-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): October 01, 2018
• Effective date (End): March 31, 2019
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Alexandra Christine Helena Frankland Sawaya
• Grantee: Mara Junqueira Carneiro
• Supervisor: Svetlana Nikolaevna Ignatova
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: Brunel University, England
• Associated to the scholarship: 16/11524-9 - Chemical composition and biological activities of ethanolic extracts and essential oils of Schinus terebinthifolius Raddi, BP.DR

Abstract

Schinus terebinthifolius Raddi is popularly known as aroeira, aroeira da praia, aroeira vermelha or pink pepper. It is a native plant to South America, amply distributed in Brazil. It has been used as a medicinal plant for many years due to its anti-inflammatory, wound-healing, antimicrobial and anti-tumor activity; and popularly applied to the treatment of respiratory tract and urinary tract infections, diarrhea, sexually transmitted diseases and ulcers.The purpose of this study is to isolate fractions from the ethanolic extracts of flowers, fruit and leaves by CCC technique. This technique presents several advantages compared to a classical column liquid chromatography (LC); and is being increasingly applied to the study of natural products with complex matrixes and used in combination with other solid phase technologies for further polishing step.On return at UNICAMP, CCC fractions or isolated compounds will be submitted to in vitro tests to establish their biological properties (antimicrobial, antioxidant and cytotoxic activities). Isolated compounds will be identified by high resolution mass spectrometry and comparison to literature, and, if enough material is obtained, nuclear magnetic resonance (NMR) 1H and 13C.