Single-cell sequencing for the study of epigenetic changes in suicide completers with history of child abuse

• Grant number: 18/11963-8
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: October 20, 2018
• End date: May 19, 2019
• Field of knowledge: Health Sciences - Medicine - Psychiatry
• Principal Investigator: Beny Lafer
• Grantee: Camila Nascimento Mantelli
• Supervisor: Gustavo Turecki
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: McGill University, Montreal, Canada
• Associated to the scholarship: 17/07089-8 - Investigating TDP-43 as neuromarker of Bipolar Disorder, BP.PD

Abstract

Children in our society are all too often subjected to physical violence, emotional deprivation and inappropriate sexual behaviors. These negative life experiences are among the strongest predictors of psychiatric illness, aggressive and violent behaviors including suicide in adulthood, with strong and lifelong effects. Recent research has suggested that early-life adversity may influence development of certain personality traits and negative mental health outcomes by changing the chemical structure of DNA. These effects, known as epigenetics were initially observed in animal studies and subsequent research suggested that they also occur in humans. The study will use a combination of high throughput techniques, including single-cell sequencing, followed by epigenome analysis, aiming to identify epigenetic changes associated with early environmental adversity.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROTENBERG, LUISA DE SIQUEIRA; BORGES-JUNIOR, RENATO GOMES; LAFER, BENY; SALVINI, ROGERIO; DIAS, RODRIGO DA SILVA. Exploring machine learning to predict depressive relapses of bipolar disorder patients. Journal of Affective Disorders, v. 295, p. 681-687, DEC 1 2021. (20/05087-0, 18/11963-8, 17/07089-8)

ROTENBERG, LUISA DE SIQUEIRA; KHAFIF, TATIANA COHAB; MISKOWIAK, KAMILLA WOZNICA; LAFER, BENY. Social cognition and bipolar disorder: pending questions and unexplored topics. Revista Brasileira de Psiquiatria, v. 44, n. 6, p. 9-pg., 2022-11-01. (17/07089-8, 18/11963-8, 20/05087-0)

TATIANA COHAB KHAFIF; ANA KLEINMAN; LUISA DE SIQUEIRA ROTENBERG; BENY LAFER. Emotion regulation and the COVID-19 pandemic: a practical guide to rehabilitating children and adolescents. Revista Brasileira de Psiquiatria, v. 44, n. 3, p. 235-236, 2021-12-06. (18/11963-8, 20/05087-0, 17/07089-8)

MAITRA, MALOSREE; NAGY, CORINA; CHAWLA, ANJALI; WANG, YU CHANG; NASCIMENTO, CAMILA; SUDERMAN, MATTHEW; THEROUX, JEAN-FRANCOIS; MECHAWAR, NAGUIB; RAGOUSSIS, JIANNIS; TURECKI, GUSTAVO. Extraction of nuclei from archived postmortem tissues for single-nucleus sequencing applications. Nature Protocols, v. 16, n. 6, p. 2788+, JUN 2021. (18/11963-8)

GABRIEL, FERNANDA C.; OLIVEIRA, MANOELA; BERK, MICHAEL; BRIETZKE, ELISA; JACKA, FELICE N.; LAFER, BENY. Nutrition and bipolar disorder: a systematic review. NUTRITIONAL NEUROSCIENCE, v. N/A, p. 15-pg., 2022-05-24. (20/05087-0, 18/11963-8, 17/07089-8)