Development of a microfluidic analytical device for on-site quantification of pesticides after spraying in plants with citrus greening disease

• Grant number: 18/14668-7
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: February 01, 2019
• End date: January 31, 2020
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Anderson Rodrigo Moraes de Oliveira
• Grantee: Daniel Blascke Carrão
• Supervisor: Charles Henry
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Colorado State University, United States
• Associated to the scholarship: 16/15680-5 - Enantioselective in vitro studies of metabolism, enzymatic inhibition and toxicity of the pesticide fipronil, BP.DR

Abstract

Greening is a citrus plant disease caused by gram-negative bacteria and transmitted by insect vectors. Citrus greening disease has a severe impact in the citrus worldwide production. The economic loss caused by this disease, both in Brazil and the United States of America, is extremely significative with great revenue losses. There are no known curative methods to combat the citrus greening disease. Its control is preventive with an aggressive removal of the infected citrus plants leading to severe economic impacts. Alternative strategies include biological and chemical control programs. Chemical control programs are based on the use of pesticides against the insect vector. However, currently employed chemical programs are not very effective as the citrus psyllids has developed resistance to the commonly used pesticides. This may be an emerging concern for the control of citrus greening disease. Therefore, the development of a new and more effective chemical control program is need and urgent. To accomplish that, it is important to understand the movement of citrus psyllids and why pesticides have little effect at slowing their spread. In this way, the evaluation of the distribution on plants of the commonly used pesticides is essential. There are several traditional analytical techniques employed for pesticides analysis in real samples, however almost none of them allows to perform the analysis in the field. Microfluidic analytical devices are a new trend in analytical determinations. Its advantages include rapid analysis, on-chip detection, low sample amount needed, low cost and the possibility to perform analysis in the field, which provides faster results. The present project aims to develop a new microfluidic analytical device for the analysis of some pesticides in the field. The developed microfluidic device will be employed to analyze samples from Florida (USA) citrus plants fields, which will allow a better understanding of the pesticide distribution in plants and will provide a better handle on managing the citrus greening disease.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARRAO, DANIEL BLASCKE; PEROVANI, ICARO SALGADO; PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; MORAES DE OLIVEIRA, ANDERSON RODRIGO. Enantioseparation of pesticides: A critical review. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 122, JAN 2020. (14/50945-4, 16/15680-5, 18/14668-7, 18/07534-4)

NOVIANA, EKA; CARRAO, DANIEL BLASCKE; PRATIWI, RIMADANI; HENRY, CHARLES S.. Emerging applications of paper-based analytical devices for drug analysis: A review. Analytica Chimica Acta, v. 1116, p. 70-90, JUN 15 2020. (18/14668-7)

JANG, ILHOON; CARRAO, DANIEL B.; MENGER, RUTH F.; MORAES DE OLIVEIRA, ANDERSON R.; HENRY, CHARLES S.. Pump-Free Microfluidic Rapid Mixer Combined with a Paper-Based Channel. ACS SENSORS, v. 5, n. 7, p. 9-pg., 2020-07-24. (18/14668-7)

CARRAO, DANIEL BLASCKE; HABENCHUS, MAISA DANIELA; PEREZ DE ALBUQUERQUE, NAYARA CRISTINA; DA SILVA, RODRIGO MOREIRA; LOPES, NORBERTO PEPORINE; MORAES DE OLIVEIRA, ANDERSON RODRIGO. In vitro inhibition of human CYP2D6 by the chiral pesticide fipronil and its metabolite fipronil sulfone: Prediction of pesticide-drug interactions. Toxicology Letters, v. 313, p. 196-204, OCT 1 2019. (16/07597-0, 14/50945-4, 16/15680-5, 18/14668-7, 18/07534-4)