Fipronil is a chiral pesticide worldwide employed in the control of insects. In Brazil, it has been used in several crops such as rice, potato, sugar cane, barley, bean, corn, soybean, among others. Despite enantiomers of chiral pesticides have the same physical-chemical properties; they may have enantioselective differences of activity, bioaccumulation, toxicity and metabolism. Although there are no significant differences in the biological activities of fipronil enantiomers in insects, enantioselective studies of toxicity and bioaccumulation of fipronil in green algae, fish and earthworms showed significant differences in the activities of the enantiomers. Enantioselective evaluations of metabolism and toxicity of this pesticide in non-target organisms, mainly humans, are extremely important for the risk assessment of this compound. The in vitro metabolism of racemic mixture of fipronil has already been evaluated. However, in vitro metabolism of each fipronil enantiomer was not individually determined. Therefore, this project aims to evaluate the metabolism of R-fipronil and S-fipronil, individually, to characterize the enzymatic kinetics and further to perform enzymatic inhibition studies as well as the evaluation of in vitro toxicity (cito- and genotoxicity) employing HepG2 culturing cells as human model.
News published in Agência FAPESP Newsletter about the scholarship:
PEREZ DE ALBUQUERQUE, NAYARA CRISTINA;
CARRAO, DANIEL BLASCKE;
HABENSCHUS, MAISA DANIELA;
MORAES DE OLIVEIRA, ANDERSON RODRIGO.
Metabolism studies of chiral pesticides: A critical review.
Journal of Pharmaceutical and Biomedical Analysis,
JAN 5 2018.
Web of Science Citations: 35.
Please report errors in scientific publications list by writing to: