Ethofumesate (ETO) is a pesticide of the class of benzofuranil herbicides widely used in beet, sprout and grass crops. The ETO has a center of asymmetry, in other words, exist as a pair of enantiomers. Studies have shown that pesticides can inhibit the cytochrome P450 (CYP450) enzymes, which are responsible for the metabolism of approximately 70% of the drugs marketed; in addition, some studies have found that the CYP450 inhibition occurred enantioselectively. Due to the presence of pesticide residues in the in water and food samples, and the scarcity of reports regarding the consequences of the interactions between pesticides and the human organism, this project proposes to evaluate whether the chiral pesticide ETO is able to inhibit, in vitro, the major CYP450 enzymes involved in drug metabolism (CYP1A2, CYP2B6, CYP2D6, CYP2C9, CYP2C19, CYP2E1 and CYP3A4/5) to further to predict pesticide-drug interaction. It is known that enantiomers may selectively interact in organisms, due to this, the enzymatic inhibition with the isolated enantiomers will be investigated to evaluate if there is difference in the inhibitory potential between them and also in comparison to the racemic mixture. The risk assessment for pesticides in this study is of great importance since the inhibition of cytochrome P450 enzymes may alter the pharmacokinetics of a particular drug causing a possible intoxication.
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