Endothelial-induced osteogenic phenotype: lessons from ultra-structural analysis of equivalent tissue

• Grant number: 18/18785-8
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Effective date (Start): October 15, 2018
• Effective date (End): December 14, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Willian Fernando Zambuzzi
• Grantee: Geórgia da Silva Feltran
• Supervisor: Alicia Ines Torres
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Research place: Universidad Nacional de Córdoba (UNC), Argentina
• Associated to the scholarship: 16/20505-8 - Endothelial tissue spheroids: acquisition, charactherization and their osteopromotor potential, BP.MS

Abstract

Bone tissue is a highly specialized tissue, harboring a set of cells that support bone formation throughout life, and its regenerative capacity is compromised during the aging process. This process of bone regeneration and repair systemically requires the synchronization of different types of cells capable of recovering the lost tissue, preserving the original aspects. In this sense, it has been demonstrated that, during osteogenesis, there is synchrony between the endothelial and osteoprogenitor cells, but little is known about the proteomic repertoire involved.Among our main objectives, exploring alternative tools to identify biological needs is our vocation and, in this sense, we characterize a three-dimensional culture of endothelial tissue, combining smooth and endothelial muscle cells capable of understanding crosstalk between these two cell phenotypes. Once characterized, we are interested in understanding the proteomic repertoire capable of conducting the maintenance of the endothelium, as well as using this functional tissue to evaluate paracrine signaling based on the endothelium capable of promoting osteogenesis (process 2016 / 20505-8). In fact, it is urgent to understand this paracrine signaling capable of guiding the differentiation of osteoblasts. In order to expand the ability of these equivalent tissues to stimulate osteogenesis, it is necessary to address the full characterization of equivalent tissues. Here, to address this question, we propose to evaluate a complete ultrastructural analysis of three-dimensional structures, also known as spheroids. (AU)