Study of transcriptomic profile in immune cells of peripheral blood during early pregnancy in beef heifers

• Grant number: 17/13994-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2018
• End date: December 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Guilherme Pugliesi
• Grantee: Cecilia Constantino Rocha
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/10606-9 - Innovations in the early pregnancy diagnosis in cattle, AP.JP
• Associated scholarship(s): 19/02995-6 - Study of the enriched-pathways in immune cells during early pregnancy and new pregnancy markers in the whole blood of bovine females, BE.EP.MS

Abstract

The identification of gestational status earlier than the current methods performed around the 30th day after insemination allows a reduction in the interval of inseminations, which can result in a shorter conception period and greater profitability to the livestock production system. The central thesis of the project is that biomarkers in the blood stimulated by the concept are detectable in early gestation. This may allow the development of an early and innovative method of diagnosing gestation in cattle. Thus, the main objective is to discover new markers of gestation in immune cells to serve as a basis for the development of molecular methods of early diagnosis of pregnancy in cattle. For this, in Experiment 1, new markers of gestational status can be identified through the transcriptomic study of blood immune cells on day 18 post-insemination. It is intended to characterize the abundance of new transcripts stimulated by the presence of the concept in polymorphonuclear (PMNs) and mononuclear (PBMCs) immune cells. Luteolysis will be detected by Doppler ultrasonography through changes in the size and vascularization of the corpus luteum (CL) and circulating concentrations of progesterone (P4) to detect cows with nonfunctional CL. The new knowledge and innovative methods proposed here will underpin the development of technologies for dairy beef cows.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA, CECILIA CONSTANTINO; DA SILVA ANDRADE, SONIA CRISTINA; DE MELO, GABRIELA DALMASO; MOTTA, IGOR GARCIA; COUTINHO, LUIZ LEHMANN; GONELLA-DIAZA, ANGELA MARIA; BINELLI, MARIO; PUGLIESI, GUILHERME. Early pregnancy-induced transcripts in peripheral blood immune cells in Bos indicus heifers. SCIENTIFIC REPORTS, v. 10, n. 1, AUG 13 2020. (17/13994-5, 15/10606-9)

ROCHA, CECILIA CONSTANTINO; DA SILVEIRA, JULIANO COELHO; FORDE, NIAMH; BINELLI, MARIO; PUGLIESI, GUILHERME. Conceptus-modulated innate immune function during early pregnancy in ruminants: a review. ANIMAL REPRODUCTION, v. 18, n. 1, p. 16-pg., 2021-01-01. (19/02995-6, 17/13994-5, 15/10606-9)

ROCHA, CECILIA CONSTANTINO; MARTINS, THIAGO; CARDOSO, BEATRIZ OLIVEIRA; SILVA, LUCIANO ANDRADE; BINELLI, MARIO; PUGLIESI, GUILHERME. Ultrasonography-accessed luteal size endpoint that most closely associates with circulating progesterone during the estrous cycle and early pregnancy in beef cows. Animal Reproduction Science, v. 201, p. 12-21, FEB 2019. (17/13994-5, 16/23964-3, 15/10606-9)

CECILIA CONSTANTINO ROCHA; JULIANO COELHO DA SILVEIRA; NIAMH FORDE; MARIO BINELLI; GUILHERME PUGLIESI. Conceptus-modulated innate immune function during early pregnancy in ruminants: a review. Animal Reproduction, v. 18, n. 1, 2021-05-10. (15/10606-9, 19/02995-6, 17/13994-5)

ROCHA, CECILIA CONSTANTINO; MARTINS, THIAGO; MELLO, BARBARA PIFFERO; DE MELLO, GABRIELA DALMASO; MOTTA, IGOR GARCIA; LEMES, KLEBER MENEGON; BINELLI, MARIO; MADUREIRA, ED HOFFMANN; PUGLIESI, GUILHERME. Comparing the effect of estradiol benzoate and 17 beta-estradiol plus progesterone on follicular turnover and development, and pregnancy outcomes in a timed artificial insemination protocol. Theriogenology, v. 192, p. 8-pg., 2022-10-15. (17/13994-5, 15/10606-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROCHA, Cecilia Constantino. Studies of early pregnancy-induced transcripts in peripheral blood immune cells in cattle. 2019. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.