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Early pregnancy diagnosis in cattle through the expression of interferon-tau stimulated genes in neutrophils

Grant number: 17/13472-9
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2017
Effective date (End): December 31, 2019
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Guilherme Pugliesi
Grantee:Gabriela Dalmaso de Melo
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:15/10606-9 - Innovations in the early pregnancy diagnosis in cattle, AP.JP
Associated scholarship(s):18/20108-4 - Use of interferon-tau stimulated genes and bovine pregnancy-associated glycoproteins to predict pregnancy loss in Nelore cows and heifers, BE.EP.MS


The early pregnancy diagnosis may allow a reduction in the interval of inseminations, resulting in a shorter calving to first service interval, a reduction in the calving interval and a positive impact on the profitability of the livestock production system. In the present study, the central thesis is based on the fact that the bovine concept can stimulate transcripts in immune cells that would serve as biomarkers in the blood and could be detectable at the beginning of gestation, thus allowing the development of an early method (before 21 days post-insemination) and innovative pregnancy diagnosis in cattle. The main objective of this study was to develop a laboratory method for the diagnosis of gestation by quantifying the abundance of interferon tau-stimulated genes (ISGs) in polymorphonuclear immune cells (PMNs; neutrophils), which are more sensitive to interferon stimulation than mononuclear immune cells (PBMCs, monocytes, lymphocytes). The first stage of the study will consist in the characterization and comparison of ISG expression in PBMCs and PMNs in the initial period of gestation (Days 12 to 20 post-insemination) in pregnant and non-pregnant heifers, and later evaluation of the viability of using the technique as early pregnancy diagnosis. In the second stage, the accuracy of the gestational diagnosis will be analyzed by quantifying the expression of ISGs in PMNs. Through the results obtained, it is expected that the new knowledge and innovative methods serve as a basis for the development of techniques for dairy and beef farming, aiming the progress of animal production. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PUGLIESI, GUILHERME; DE MELO, GABRIELA DALMASO; ATAIDE, JR., GILMAR ARANTES; GONTIJO PELLEGRINO, CARLOS AUGUSTO; SILVA, JULIO BARBOZA; ROCHA, CECILIA CONSTANTINO; MOTTA, IGOR GARCIA; MORAES VASCONCELOS, JOSE LUIZ; BINELLI, MARIO. Use of Doppler ultrasonography in embryo transfer programs: feasibility and field results. ANIMAL REPRODUCTION, v. 15, n. 3, p. 239-246, . (17/18613-0, 16/23964-3, 15/10606-9, 17/13472-9)
PUGLIESI, GUILHERME; DE MELO, GABRIELA DALMASO; SILVA, JULIO BARBOZA; CARVALHEDO, ALEXANDRE SARDINHA; LOPES, EVERTON; DE SIQUEIRA FILHO, EMIVALDO; SILVA, LUCIANO ANDRADE; BINELLI, MARIO. Use of color-Doppler ultrasonography for selection of recipients in timed-embryo transfer programs in beef cattle. Theriogenology, v. 135, p. 73-79, . (16/23964-3, 15/10606-9, 17/13472-9)
DE MELO, GABRIELA DALMASO; MELLO, BARBARA PIFFERO; FERREIRA, CATIA APARECIDA; SOUTO GODOY FILHO, CARLOS ALBERTO; ROCHA, CECILIA CONSTANTINO; SILVA, AMANDA GUIMARAES; REESE, SYDNEY TAYLOR; MADUREIRA, ED HOFFMANN; POHLER, KY GARRETT; PUGLIESI, GUILHERME. Applied use of interferon-tau stimulated genes expression in polymorphonuclear cells to detect pregnancy compared to other early predictors in beef cattle. Theriogenology, v. 152, p. 94-105, . (17/13472-9, 18/20108-4, 15/10606-9)
MELO, G. D.; PINTO, L. M. F.; ROCHA, C. C.; MOTTA, I. G.; SILVA, L. A.; DA SILVEIRA, J. C.; GONELLA-DIAZA, A. M.; BINELLI, M.; PUGLIESI, G.. Type I interferon receptors and interferon-tau-stimulated genes in peripheral blood mononuclear cells and polymorphonuclear leucocytes during early pregnancy in beef heifers. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 32, n. 11, p. 953-966, . (17/13472-9, 15/10606-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MELO, Gabriela Dalmaso de. Early pregnancy diagnosis in beef cattle through the expression of Interferon-tau stimulated genes in peripheral blood polymorphonuclear cells. 2019. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.

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