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Elaboration of a recombinant multicomponent chimeric vaccine based on epitopes of tick proteins Rhipicephalus microplus

Grant number: 18/17994-2
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2018
Effective date (End): January 31, 2023
Field of knowledge:Biological Sciences - Immunology - Applied Immunology
Principal Investigator:Beatriz Rossetti Ferreira
Grantee:Mayra Larissa Brunato
Host Institution: Escola de Enfermagem de Ribeirão Preto (EERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:15/09683-9 - Development of a recombinant multicomponent chimeric vaccine based in protein epitopes from Rhipicephalus microplus ticks, AP.TEM


Ticks are important transmitters of diseases to humans and animals and promote losses in the cattle production sector, besides causing significant owners of dogs. The treatment of infestations can be done with acaricides, however the selection of resistant parasites and environmental contamination point to the need for other control methods. In trade there are only vaccines against bovine ticks, which protection among bovine herds, as well as to induce immune response of little memory lasting, indicating the need to develop more efficient vaccines. In this project we propose construct a recombinant protein composed of epitopes derived from R.microplus selected by the SPOT peptide synthesis technique. Initially, the fifteen proteins selected recombinants will be produced and evaluated in relation to their mice against infestation challenge by R. sanguineus ticks. Proteins that promote reduction of the biological and reproductive indexes of ticks will be used for inoculation of dogs and bovine animals, and the immune sera from these animals will be used for the mapping of epitopes bySPOT peptide synthesis technique. Thus, it will be identified which protein regions induce immunity in both animal species that will be targeted by the chimeric vaccine. Finally, the sequences of reagent epitopes will be used for the synthesis of a recombinant chimeric protein that allows the expression of the epitopes in a single fused structure. The multicomponent chimeric protein recombinant will be used for the immunization of mice, which will subsequently bea challenge infestation with R. sanguineus ticks in order to evaluate its efficacy as a vaccine and study the humoral and cellular response profile. Finally, in case the chimeric protein is efficient inmice will be conducted an immunization experiment in cattle and dogs for challenge test with ticks and subsequent analysis of the humoral and cellular immune response.

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