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Development of a recombinant multicomponent chimeric vaccine based in proteins epitopes from Rhipicephalus microplus ticks

Grant number: 14/11010-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2014
Effective date (End): October 31, 2018
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Beatriz Rossetti Ferreira
Grantee:Andressa Fisch
Host Institution: Escola de Enfermagem de Ribeirão Preto (EERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):17/21401-4 - Seeking vaccine antigen epitopes presented by BoLA-DR for development of a multi epitope-based vaccine against R. microplus tick infestations, BE.EP.DR


Ticks are important vectors of diseases to humans and animals and promote significant losses in cattle production sector. The treatment of infestations can be done with acaricides, but the selection of resistant parasites and environmental contamination show the need for other methods of control. The vaccines currently available against ticks show variable outcome of protection between the cattle and little lasting memory immune response, indicating the need to develop more efficient vaccines. In this project we propose to carry out the construction of a recombinant protein consisting of epitopes derived from proteins of R. microplus selected by SPOT peptide synthesis technique. Initially, Rm40, Rm76, Rm239, Rm1756 and Rm2879 proteins will be produced and evaluated with regard to its potential to protect mice against challenge by ticks R. sanguineus. The proteins that promote decrease in biological and reproductive rates of ticks will be used for inoculation of dogs and cattle, and the immune sera of these animals will be used for epitope mapping by SPOT technique, to identify regions which induce immunity in the species-target of final product. Finally, the sequences of the reagentes epitopes will be used for the synthesis of a recombinant chimeric protein allowing expression of the fused epitopes in a single structure. The multicomponent recombinant chimeric protein will be used to immunize mice that subsequently will undergo a challenge infestation with R. sanguineus ticks in which his activity as a vaccine will be evaluated. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FISCH, Andressa. Recombinant multicomponent vaccine based on antigens secreted by Rhipicephalus microplus induces protective immunity against ticks in cattle and dogs. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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