Gastroprotective effect of vanillin in experimental induced gastric ulcer

• Grant number: 18/11484-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2018
• End date: August 31, 2019
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Ariane Leite Rozza
• Grantee: Murilo Piologo Ciciliato
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The gastric ulcer condition is determined by a deep erosion in the stomach's wall that penetrates throughout the mucosa thickness. The wound is induced by a disequilibrium between gastroprotective and gastrodestructive factors. Nowadays plant derived natural products have been studied for their capacity to affect the disequilibirum in order to induce anti-inflammatory cytokines production and reduce pro-inflammatory cytokines concentration, beyond supressing free radicals production. As an alternative for conventional medicines vanillin arises. It is a substance found in orchid and used as flavouring that also presents several biological activities already proved by recent studies. The present study proposes to verify vanilin's gastroprotective activity and it's influence over ethanol-induced gastric inflammation. For this purpose male Wistar rats (n=8) will be undergoing gastric ulcer induction by absolute ethanol, after oral administration of vanilin (25, 50 and 100 mg/kg), carbnoxolone (100 mg/kg, positive control) or vehicle (8 tween 80, negative control). After euthanasia, the ulcer area wll be calculated and compared between treatment groups. Fragments of the stomach will be intended for histological analysis trough hematoxylin-eosin and acid-Schiff staining. Other fragments will be intended for neutrophilic infiltration detection (MPO activity), anti-inflammatory effects (level of cytokines TNF-±, IL-1-², IL-6 e IL-10) and plasmatic concentration of nitrite, besides immunohistochemistry reaction for NK-KB (inflammatory process related gene transcription factor).

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

CICILIATO, MURILO PIOLOGO; DE SOUZA, MATHEUS CHIARADIA; TARRAN, CAROLINA MENDES; TIRONI DE CASTILHO, ANA LAURA; VIEIRA, ANA JULIA; ROZZA, ARIANE LEITE. Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation. PHARMACEUTICS, v. 14, n. 4, p. 11-pg., 2022-04-01. (18/11484-2)