The role of the hydroalcoholic extract from the leaves of Eugenia punicifolia (Kunth) DC on experimental peptic ulcer disease: characterization of mechanisms of action anti-inflammatory, healing and antiapoptotic.

• Grant number: 15/14797-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): December 01, 2015
• Effective date (End): February 28, 2018
• Field of knowledge: Biological Sciences - Pharmacology - Ethnopharmacology
• Principal Investigator: Clélia Akiko Hiruma Lima
• Grantee: Larissa Lucena Périco
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

As subproject of the thematic project Biota entitled "Phytotherapics standardized for the treatment of chronic diseases" this work aims to characterize the mechanisms involved in the antiulcerogenic action of the hydroalcoholic extract from leaves of Eugenia punicifolia (Kunth) DC (HEEP). From its popular indication for the treatment of wounds and inflammation, and based on previous results about their gastroprotective and anti-inflammatory effects in experiments in vivo, the aim of this subproject is to characterize the mechanisms involved in the healing action of HEEP to combat ulcerogenic factors such as: absolute ethanol, acetic acid, non-steroidal anti-inflammatory drugs and injuries derived from the process of ischemia-reperfusion. It is intended to characterize the main mechanisms involved in the curative activity of HEEP. For this, we will develop experimental models in which will be initially implanted gastric or duodenal lesions in animals and thereafter will be treated with EHEP for periods that can vary from 1 to 14 days of treatment. We will analyze, in different experimental groups, macroscopic lesion area, biochemical parameters such as antioxidant (superoxide dismutase, reduced glutathione, catalase, myeloperoxidase, thioredoxin, lipid peroxidation), anti-inflammatories (TNF-±lfa, IL-1beta, IL-6, IFN-gama and IL-10) and protein and gene expression of factors involved in the wound healing process (vascular endothelial growth factor and epithelial, cyclooxygenase 1 and 2, metalloproteinases 2 and 9, pro-apoptotic markers - caspase-3, caspase-8, caspase-9 and anti-apoptotic, Bcl-2). In the experiments described above, will be obtained parameters for analysis of possible toxic effects of HEEP through the evolution of body weight, lethality (death) and analysis of macroscopic organs and biochemical of plasma. From the evaluation of pharmacological effects of HEEP in healing of peptic ulcers, it is intended to offer scientific subsidies to the future incorporation of this species as a new option therapy of phytotherapic for the treatment of peptic ulcer disease.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PERICO, LARISSA LUCENA; EMILIO-SILVA, MAYCON TAVARES; OHARA, RIE; RODRIGUES, VINICIUS PEIXOTO; BUENO, GABRIELA; BARBOSA-FILHO, JOSE MARIA; ROCHA, LUCIA REGINA MACHADO DA; BATISTA, LEONIA MARIA; HIRUMA-LIMA, CLELIA AKIKO. Systematic Analysis of Monoterpenes: Advances and Challenges in the Treatment of Peptic Ulcer Diseases. BIOMOLECULES, v. 10, n. 2, FEB 2020. (18/10935-0, 18/09873-0, 15/14797-3)

OHARA, RIE; PERICO, LARISSA LUCENA; RODRIGUES, VINICIUS PEIXOTO; BUENO, GABRIELA; ZANATTA, ANA CAROLINE; DOS SANTOS, LOURDES CAMPANER; VILEGAS, WAGNER; CONSTATINO, FLAVIA BESSI; JUSTULIN, LUIS ANTONIO; HIRUMA-LIMA, CLELIA AKIKO. Terminalia catappa L. infusion accelerates the healing process of gastric ischemia-reperfusion injury in rats. Journal of Ethnopharmacology, v. 256, JUN 28 2020. (15/14797-3)

PERICO, LARISSA LUCENA; RODRIGUES, VINICIUS PEIXOTO; OHARA, RIE; BUENO, GABRIELA; ALFIERI NUNES, VANIA VASTI; DOS SANTOS, RAQUEL DE CASSIA; LIMA CAMARGO, ANA CAROLINA; JUSTULIN JUNIOR, LUIS ANTONIO; DE ANDRADE, SERGIO FALONI; BISPO STEIMBACH, VIVIANE MIRANDA; et al. Sex-specific effects of Eugenia punicifolla extract on gastric ulcer healing in rats. WORLD JOURNAL OF GASTROENTEROLOGY, v. 24, n. 38, p. 4369-4383, OCT 14 2018. (09/52237-9, 15/14797-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PÉRICO, Larissa Lucena. Investigation of the role of sex hormones in healing activity of the hydroalcoholic extract from the leaves of Eugenia punicifolia on experimental peptic ulcer disease.. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.