Influence of the HIPPO signaling pathway on the segregation between the inner cell mass and the trophectoderm in bovine embryos

• Grant number: 18/18924-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2018
• End date: November 30, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcelo Demarchi Goissis
• Grantee: Caroline Pereira da Costa
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 17/09576-3 - Studies on cell differentiation mechanisms during early bovine embryo development, AP.JP

Abstract

The first cell differentiation event in mammals consists in the segregation between the inner cell mass (ICM) and the trophectoderm (TE). In mice, HIPPO signaling pathway plays an important role in the control of genes that define ICM/TE segregation. In bovine, there is evidence that the same genes do not participate in a similar manner, but HIPPO pathway may still contribute to this biological event. The present project aims to test the hypothesis that LATS1/2 activity is required for ICM differentiation in bovine embryos. We intend to eliminate LATS through gene editing using CRISPR/Cas9 in in vitro produced embryos. Embryos will be cultured for assessment of blastocyst formation rates, cell distribution, gene and protein expression. We expect to inhibit ICM due to absence of LATS-mediated HIPPO signaling. Consequently, this project would provide a better comprehension in the mechanisms of ICM/TE segregation, which can be useful to understand failures in the vitro production processes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS ANJOS, S. A. A.; DA COSTA, C. P.; ASSUMPCAO, M. E. O. A.; VISINTIN, J. A.; GOISSIS, M. D.. Inhibition of apical domain formation does not block blastocyst development in bovine embryos. REPRODUCTION FERTILITY AND DEVELOPMENT, JUN 2021. (17/25574-0, 17/09576-3, 18/08285-8, 18/18924-8)

LUEDKE, FELIPE EDUARDO; DA COSTA, CAROLINE PEREIRA; MENDES, CAMILLA MOTA; DOS SANTOS HAMILTON, THAIS ROSE; MILAZZOTTO, MARCELLA PECORA; ASSUMPCAO, MAYRA E. O. A.; GOISSIS, MARCELO DEMARCHI. Effects of fetal bovine serum on trophectoderm and primitive endoderm cell allocation of in vitro-produced bovine embryos. ZYGOTE, v. 31, n. 1, p. 7-pg., 2022-10-24. (18/18924-8, 17/09576-3, 19/03014-9, 17/25574-0)

DOS ANJOS, S. A. A.; DA COSTA, C. P.; ASSUMPCAO, M. E. O. A.; VISINTIN, J. A.; GOISSIS, M. D.. Inhibition of apical domain formation does not block blastocyst development in bovine embryos. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 33, n. 10, p. 665-673, AUG 11 2021. (17/09576-3, 18/08285-8, 18/18924-8, 17/25574-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

COSTA, Caroline Pereira da. Influence of HIPPO signaling pathway on segregation between internal cell mass and trophectoderm in bovine embryos. 2020. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.